DDX20 antibody (AA 368-548)

Catalog No. ABIN108548

This Mouse Monoclonal antibody specifically detects DDX20 in WB, ELISA, IHC, IF, IP, FACS and ICC. It exhibits reactivity toward Human, Mouse, Rat and Hamster and has been mentioned in 8+ publications.

Quick Overview for DDX20 antibody (AA 368-548) (ABIN108548)

Target: DDX20 (DEAD (Asp-Glu-Ala-Asp) Box Polypeptide 20 (DDX20))

Reactivity: Human, Mouse, Rat, Hamster

Host: Mouse

Clonality: Monoclonal

Conjugate: This DDX20 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF), Immunoprecipitation (IP), Flow Cytometry (FACS), Immunocytochemistry (ICC)

Clone: 12H12

Product Details anti-DDX20 Antibody

Binding Specificity: AA 368-548

Purification: Protein A

Immunogen: Recombinant 6His-tag COOH-terminal domain of Gemin3 (amino acids 368-548)

Isotype: IgG1

Application Details

Application Notes: Optimal antibody dilution should be determined by titration, however as a guideline try:WB: Use at an assay dependent dilution. Detects a band of approximately 105 kDa (predicted molecular weight: 99 kDa).Flow Cyt: Use 1 μg for 106 cells.ICC/IF: 1/50.

Comment:

Myeloma, fusion partners: SP2/0,

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Purified antibody (from supernatant) containing PBS 0.1 % sodium azide,

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

References for anti-DDX20 antibody (ABIN108548)

Mahmoudi, Henriksson, Weibrecht, Smith, Söderberg, Strömblad, Wiman, Farnebo: "WRAP53 is essential for Cajal body formation and for targeting the survival of motor neuron complex to Cajal bodies." in: PLoS biology, Vol. 8, Issue 11, pp. e1000521, (2010) (PubMed).

Bradrick, Gromeier: "Identification of gemin5 as a novel 7-methylguanosine cap-binding protein." in: PLoS ONE, Vol. 4, Issue 9, pp. e7030, (2009) (PubMed).

Castelló, Izquierdo, Welnowska, Carrasco: "RNA nuclear export is blocked by poliovirus 2A protease and is concomitant with nucleoporin cleavage." in: Journal of cell science, Vol. 122, Issue Pt 20, pp. 3799-809, (2009) (PubMed).

Walker, Rajendra, Saieva, Fuentes, Pellizzoni, Matera: "SMN complex localizes to the sarcomeric Z-disc and is a proteolytic target of calpain." in: Human molecular genetics, Vol. 17, Issue 21, pp. 3399-410, (2008) (PubMed).

Morency, Sabra, Catez, Texier, Lomonte: "A novel cell response triggered by interphase centromere structural instability." in: The Journal of cell biology, Vol. 177, Issue 5, pp. 757-68, (2007) (PubMed).

Hamamoto, Nishitsuji, Amagasa, Kannagi, Masuda: "Identification of a novel human immunodeficiency virus type 1 integrase interactor, Gemin2, that facilitates efficient viral cDNA synthesis in vivo." in: Journal of virology, Vol. 80, Issue 12, pp. 5670-7, (2006) (PubMed).

Zhang, Xing, Rossoll, Wichterle, Singer, Bassell: "Multiprotein complexes of the survival of motor neuron protein SMN with Gemins traffic to neuronal processes and growth cones of motor neurons." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 26, Issue 33, pp. 8622-32, (2006) (PubMed).

Charroux, Pellizzoni, Perkinson, Shevchenko, Mann, Dreyfuss: "Gemin3: A novel DEAD box protein that interacts with SMN, the spinal muscular atrophy gene product, and is a component of gems." in: The Journal of cell biology, Vol. 147, Issue 6, pp. 1181-94, (2000) (PubMed).

Target Details for DDX20

Target: DDX20 (DEAD (Asp-Glu-Ala-Asp) Box Polypeptide 20 (DDX20))

Alternative Name: Gemin 3

Background: Synonyms:
Component of gems 3 antibody,DDX 20 antibody,DDX20 antibody,DDX20_HUMAN antibody,DEAD (Asp Glu AlaAsp) box polypeptide 20 antibody,DEAD (Asp-Glu-Ala-Asp) box polypeptide 20 antibody,DEAD box protein 20antibody,DEAD box protein DP 103 antibody,DEAD box protein DP103 antibody,DEAD-box protein DP103antibody,DEAD/H (Asp Glu Ala Asp/His) box polypeptide 20 103kD antibody,DEAD/H (Asp Glu Ala Asp/His) boxpolypeptide 20 antibody,DEAD/H BOX 20 antibody,DKFZP434H052 antibody,DP 103 antibody,DP103 antibody,Gemin-3antibody,Gemin3 antibody,Probable ATP dependent RNA helicase DDX20 antibody,Probable ATP-dependent RNA helicaseDDX20 antibody,SMN interacting protein antibody

Gene ID: 66603

OMIM: 606168

Pathways: Ribonucleoprotein Complex Subunit Organization