HNRNPL antibody

Catalog No. ABIN108588

This anti-HNRNPL antibody is a Mouse Monoclonal antibody detecting HNRNPL in WB, ELISA, IF, IHC (p), IP, FACS, ICC and IHC (fro). Suitable for Human, Mouse, Rat and Newt. This Primary Antibody has been cited in 9+ publications.

Quick Overview for HNRNPL antibody (ABIN108588)

Target: HNRNPL (Heterogeneous Nuclear Ribonucleoprotein L (HNRNPL))

Reactivity: Human, Mouse, Rat, Newt

Host: Mouse

Clonality: Monoclonal

Conjugate: This HNRNPL antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Flow Cytometry (FACS), Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro))

Clone: 4D11

Product Details anti-HNRNPL Antibody

Purification: Protein A

Immunogen: Human hnRNP proteins (from HeLa cells) purified by affinity chromatography on ssDNA agarose

Isotype: IgG1

Application Details

Application Notes: Optimal antibody dilution should be determined by titration, however as a guideline try, IHC-P: Use a concentration of 1 μg/mL. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.WB: 1/2000. Detects a band of approximately 64 kDa (predicted molecular weight: 64 kDa).Flow Cyt: Use 1 μg for 106 cells.

Comment:

Myeloma, fusion partners: SP2/0

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Purified antibody (from supernatant) containing PBS 0.1 % sodium azide,

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

References for anti-HNRNPL antibody (ABIN108588)

Peddigari, Li, Rabe, Martin: "hnRNPL and nucleolin bind LINE-1 RNA and function as host factors to modulate retrotransposition." in: Nucleic acids research, Vol. 41, Issue 1, pp. 575-85, (2012) (PubMed).

Motta-Mena, Smith, Mallory, Jackson, Wang, Lynch: "A disease-associated polymorphism alters splicing of the human CD45 phosphatase gene by disrupting combinatorial repression by heterogeneous nuclear ribonucleoproteins (hnRNPs)." in: The Journal of biological chemistry, Vol. 286, Issue 22, pp. 20043-53, (2011) (PubMed).

Heyd, Lynch: "Phosphorylation-dependent regulation of PSF by GSK3 controls CD45 alternative splicing." in: Molecular cell, Vol. 40, Issue 1, pp. 126-37, (2010) (PubMed).

Wang, Cambi: "Heterogeneous nuclear ribonucleoproteins H and F regulate the proteolipid protein/DM20 ratio by recruiting U1 small nuclear ribonucleoprotein through a complex array of G runs." in: The Journal of biological chemistry, Vol. 284, Issue 17, pp. 11194-204, (2009) (PubMed).

Niu, Terasaki, Komatsu, Noguchi, Shichijo, Itoh: "Identification of peptides applicable as vaccines for HLA-A26-positive cancer patients." in: Cancer science, Vol. 100, Issue 11, pp. 2167-74, (2009) (PubMed).

Jia, Liu, Tao, Kruhlak, Guo, Meyers, Baker, Zheng: "Control of the papillomavirus early-to-late switch by differentially expressed SRp20." in: Journal of virology, Vol. 83, Issue 1, pp. 167-80, (2008) (PubMed).

Venables, Koh, Froehlich, Lapointe, Couture, Inkel, Bramard, Paquet, Watier, Durand, Lucier, Gervais-Bird, Tremblay, Prinos, Klinck, Elela, Chabot: "Multiple and specific mRNA processing targets for the major human hnRNP proteins." in: Molecular and cellular biology, Vol. 28, Issue 19, pp. 6033-43, (2008) (PubMed).

Rothrock, House, Lynch: "HnRNP L represses exon splicing via a regulated exonic splicing silencer." in: The EMBO journal, Vol. 24, Issue 15, pp. 2792-802, (2005) (PubMed).

Piñol-Roma, Swanson, Gall, Dreyfuss: "A novel heterogeneous nuclear RNP protein with a unique distribution on nascent transcripts." in: The Journal of cell biology, Vol. 109, Issue 6 Pt 1, pp. 2575-87, (1990) (PubMed).

Target Details for HNRNPL

Target: HNRNPL (Heterogeneous Nuclear Ribonucleoprotein L (HNRNPL))

Alternative Name: hnRNP L

Background: Synonyms:
D830027H13Rik antibody,FLJ35509 antibody,Heterogeneous nuclear ribonucleoprotein L antibody,hnRNPLantibody,hnRPL antibody,P/OKcl.14 antibody

Gene ID: 3191, 15388, 80846

OMIM: 603083

UniProt: P31943