HNRNPA1 antibody

Catalog No. ABIN108610

This anti-HNRNPA1 antibody is a Mouse Monoclonal antibody detecting HNRNPA1 in WB, ELISA, IF, IP, ICC, IHC (p) and FACS. Suitable for Human and Mouse. This Primary Antibody has been cited in 10+ publications.

Quick Overview for HNRNPA1 antibody (ABIN108610)

Target: HNRNPA1 (Heterogeneous Nuclear Ribonucleoprotein A1 (HNRNPA1))

Reactivity: Human, Mouse

Host: Mouse

Clonality: Monoclonal

Conjugate: This HNRNPA1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunoprecipitation (IP), Immunocytochemistry (ICC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Flow Cytometry (FACS)

Clone: 9H10

Product Details anti-HNRNPA1 Antibody

Purification: Protein A

Immunogen: Full length hnRNP-A1 native protein (partially purified) from HeLa cells (human)

Isotype: IgG2b

Application Details

Application Notes: Optimal antibody dilution should be determined by titration, however as a guideline try,Flow Cyt: Use 1 μg for 106 cells.IHC-P: Use a concentration of 1 μg/mL. Perform heat mediated antigen retrieval before commencing with IHC staining protocolWB: Use at 1:2000-1:4000. Detects a band of approximately 34 kDa (predicted molecular weight: 38 kDa)IP: Use at an assay dependent dilution. This antibody does not IP the hnRNP complex

Comment:

Myeloma, fusion partners: Balb/c Sp2/0 cell line

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Purified antibody (from supernatant) containing PBS 0.1 % sodium azide,

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

References for anti-HNRNPA1 antibody (ABIN108610)

Woolley, Algie, Samuel, Harfoot, Wiles, Hung, Tan, Hains, Valova, Huschtscha, Royds, Perez, Yoon, Cohen, Robinson, Bay, Lasham, Braithwaite: "Prognostic association of YB-1 expression in breast cancers: a matter of antibody." in: PLoS ONE, Vol. 6, Issue 6, pp. e20603, (2011) (PubMed).

Beauchamp, Nassif, Hillock, van der Giessen, von Roretz, Jasmin, Gallouzi: "The cleavage of HuR interferes with its transportin-2-mediated nuclear import and promotes muscle fiber formation." in: Cell death and differentiation, Vol. 17, Issue 10, pp. 1588-99, (2010) (PubMed).

Read, Digard: "Individual influenza A virus mRNAs show differential dependence on cellular NXF1/TAP for their nuclear export." in: The Journal of general virology, Vol. 91, Issue Pt 5, pp. 1290-301, (2010) (PubMed).

Rosenberger, De-Castro Arce, Langbein, Steenbergen, Rösl: "Alternative splicing of human papillomavirus type-16 E6/E6* early mRNA is coupled to EGF signaling via Erk1/2 activation." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 107, Issue 15, pp. 7006-11, (2010) (PubMed).

Sellier, Rau, Liu, Tassone, Hukema, Gattoni, Schneider, Richard, Willemsen, Elliott, Hagerman, Charlet-Berguerand: "Sam68 sequestration and partial loss of function are associated with splicing alterations in FXTAS patients." in: The EMBO journal, Vol. 29, Issue 7, pp. 1248-61, (2010) (PubMed).

Kocgozlu, Lavalle, Koenig, Senger, Haikel, Schaaf, Voegel, Tenenbaum, Vautier: "Selective and uncoupled role of substrate elasticity in the regulation of replication and transcription in epithelial cells." in: Journal of cell science, Vol. 123, Issue Pt 1, pp. 29-39, (2009) (PubMed).

Lieberthal, Kaminsky, Parkhurst, Tanese: "The role of YY1 in reduced HP1alpha gene expression in invasive human breast cancer cells." in: Breast cancer research : BCR, Vol. 11, Issue 3, pp. R42, (2009) (PubMed).

Kashima, Rao, David, Manley: "hnRNP A1 functions with specificity in repression of SMN2 exon 7 splicing." in: Human molecular genetics, Vol. 16, Issue 24, pp. 3149-59, (2007) (PubMed).

van der Giessen, Gallouzi: "Involvement of transportin 2-mediated HuR import in muscle cell differentiation." in: Molecular biology of the cell, Vol. 18, Issue 7, pp. 2619-29, (2007) (PubMed).

Burd, Dreyfuss: "RNA binding specificity of hnRNP A1: significance of hnRNP A1 high-affinity binding sites in pre-mRNA splicing." in: The EMBO journal, Vol. 13, Issue 5, pp. 1197-204, (1994) (PubMed).

Target Details for HNRNPA1

Target: HNRNPA1 (Heterogeneous Nuclear Ribonucleoprotein A1 (HNRNPA1))

Alternative Name: hnRNP A1 

Background: Synonyms:
HNRNPA 1 antibody,Helix destabilizing protein antibody,Helix-destabilizing proteinantibody,Heterogeneous nuclear ribonucleoprotein A1 antibody,Heterogeneous nuclear ribonucleoprotein A1B proteinantibody,Heterogeneous nuclear ribonucleoprotein B2 protein antibody,Heterogeneous nuclear ribonucleoprotein coreprotein A1 antibody,hnRNP A1 antibody,hnRNP core protein A1 antibody,HNRNPA1 antibody,HNRPA1antibody,MGC102835 antibody,Nuclear ribonucleoprotein particle A1 protein antibody,ROA1_HUMANantibody

Gene ID: 3178, 15382

OMIM: 164017

UniProt: P09651, P49312