Carcinoembryonic Antigen-Related Cell Adhesion Molecule 7 (CEACAM7) antibody

Antigen: Carcinoembryonic Antigen-Related Cell Adhesion Molecule 7 (CEACAM7)

Synonyms: CEA, CGM2, CEACAM7

Clonality: Monoclonal (BAC2)

Host: Mouse

Reactivity: Human

Application: Flow Cytometry (FACS), Cell-ELISA (cELISA)

Catalog no.: ABIN108732

Additional Information

Alternative name: CEACAM7 (CGM2)

Immunogen: immunisation with a CEACAM7/Fc fusion protein

Format: Purified

Isotype: IgG1

Clone: BAC2

Description: CEA-related cell adhesion molecule 7 (CEACAM7, CGM2) belongs to the carcinoembryonic antigen (CEA) family (1). It encodes a glycosyl phosphatidylinositol (GPI)-linked glycoprotein which is only found on the apical surface of highly differentiated epithelial cells adult colon and on a small epithelial cell population in pancreatic ducts (2). CEACAM7 expression is completely lost upon malignant transformation already in hyperplastic polyps and early adenomas as well as in adenocarcinomas of the colon (2). Like all members of the CEACAM family, it consists of a single N domain, with structural homology to the immunoglobulin variable domains, followed by one immunoglobulin constantlike A domain.

Specificity: Anti-human CEACAM7 (CGM2)

Application Details

Application Notes: Flow cytometry: 1.2 ug/10^6 cells. CELISA: 1:200 - 1:400.

Concentration: 1mg/ml

Purification: Protein G

Buffer: phosphate buffered saline, pH 7.2

Storage: Short term: 2°C - 8°C. Long term: -20°C (avoid repeated freezing and thawing)

Research Area: Cancer

Restrictions: For Research Use only

Images

Specificity testing of BAC2. Members of the CEACAM family were expressed on BOSC cells after transient transfection with expression vectors containing either the cDNA of CEACAM1, 5, 6, 7, 8 or a recombinant transmembrane- anchored PSG1 fusion protein. Re

FACS analysis of BOSC23 cells using BAC2.BOSC23 cells were transiently transfected with an expression vectorencoding either CEACAM7 (red curve) or an irrelevant protein (control transfectant). Binding of BAC2 was detected with a PE conjugatedsecondary an

Publications

Publications: Schoelzel, Zimmermann, Schwarzkopf et al.: "Carcinoembryonic antigen family members CEACAM6 and CEACAM7 are differentially expressed in normal tissues and oppositely deregulated in hyperplastic colorectal polyps and early adenomas." in: The American journal of pathology, Vol. 156, Issue 2, pp. 595-605, 2000 (PubMed).

Kilic, Oliveira-Ferrer, Neshat-Vahid et al.: "Lymphatic reprogramming of microvascular endothelial cells by CEA-related cell adhesion molecule-1 via interaction with VEGFR-3 and Prox1." in: Blood, Vol. 110, Issue 13, pp. 4223-33, 2007 (PubMed).