Carcinoembryonic Antigen-Related Cell Adhesion Molecule 5 (CEACAM5) antibody

Antigen: Carcinoembryonic Antigen-Related Cell Adhesion Molecule 5 (CEACAM5)

Synonyms: CEA, CD66e, DKFZp781M2392, Psg30, 1600029H12Rik, CEACAM5, CEACAM

Clonality: Monoclonal (26-5-1)

Host: Mouse

Reactivity: Human

Application: Flow Cytometry (FACS), Immunohistochemistry (IHC), Cell-ELISA (cELISA), Western Blotting (WB), ELISA

Catalog no.: ABIN108734

Additional Information

Alternative name: CEACAM5 (CEA, CD66e)

Immunogen: Immunisation with extracted protein of CEACAM5. Selection based on recognition of the complete native protein expressed on transfected mammalian cells

Format: Purified

Isotype: IgG2a

Clone: 26-5-1

Description: CEACAM5 (CEA-related cell adhesion molecule 5, CEA) belongs to the carcinoembryonic antigen (CEA) gene family (2). It encodes a glycosyl phosphatidyl inositol (GPI)-linked glycoprotein with a Mr of 180,000-200,000 which is most strongly expressed on epithelial cells of the fetal and adult colon and to a minor extent on epithelial cells of the stomach and sweat glands, squamous epithelial cell of the tongue, esophagus and cervix. CEACAM5 is used as a tumor marker for early detection of recurrent disease due its expression in adenocarcinomas of the colon, lung, breast, stomach and pancreas and in mucinous ovarian carcinomas (2). Like all members of the CEACAM family, it consists of a single N domain, with structural homology to the immunoglobulin variable domains, followed by six immunoglobulin constant-like A (A1, A2, A3) and B domains (B1, B2, B3).

Specificity: Anti-human CEACAM5 (CEA, CD66e)

Application Details

Application Notes: Flow cytometry (1.2 ug/10^6 cells), cell-ELISA (1:200), ELISA (1:200 - 1:400), Western blots (4ug/ml) and immunohistology on cryosections (1-2 ug/10^6 cells).

Concentration: 1 mg/ml

Buffer: phosphate buffered saline, pH 7.2

Storage: Short term: 2°C - 8°C. Long term: -20°C (avoid repeated freezing and thawing)

Research Area: Receptors, Cancer, Receptors, Cell/Tissue Markers, Cancer

Restrictions: For Research Use only

Images

Specificity testing of 26/5/1. Members of the CEA family were expressed on BOSC cells after transient transfection with expression vectors containing either the cDNA of CEACAM1, 5, 6 or 8. Recognition of CEACAM3 and of a recombinant transmembrane-anchore

FACS analysis of BOSC23 cells using 26/5/1. BOSC23 cells were transiently transfected with an expres-sion vector encoding either CEACAM5 (red curve) or an irrelevant protein (control transfectant). Binding of 26/5/1 was detected witha PE conjugated secon

Publications

Publications: Manosroi, von Kleist, Manosroi et al.: "Thermo-stability and antitumor activity on colon cancer cell lines of monoclonal anti-CEA antibody-saporin immunotoxin." in: Journal of Korean medical science, Vol. 7, Issue 2, pp. 128-35, 1992 (PubMed).

Hammarstrom, Shively, Paxton et al.: "Antigenic sites in carcinoembryonic antigen." in: Cancer research, Vol. 49, Issue 17, pp. 4852-8, 1989 (PubMed).

Strobel ES, Strobel HG, Bross KJ et al.: "Effects of human bone marrow stroma on the growth of human tumor cells." in: Cancer Res, Vol. 49, Issue 4, pp. 1001-7, 1989 (PubMed).

Grunert, AbuHarfeil, Schwarz et al.: "Two CEA and three NCA species, although distinguishable by monoclonal antibodies, have nearly identical peptide patterns." in: International journal of cancer. Journal international du cancer, Vol. 36, Issue 3, pp. 357-62, 1985 (PubMed).