Twinfilin, Actin-Binding Protein, Homolog 1 (Drosophila) (TWF1) (AA 335-384) antibody

Details for Product No. ABIN1098116
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Antigen
Synonyms CG3771, Dmel\\CG3771, EG:9D2.3, DKFZp469O1925, A6, PTK9, ptk9, Ptk9, twinfilin, twf1, wu:fd02b03, zgc:65922
Epitope
AA 335-384
(13), (5), (4), (3), (2), (2), (1), (1), (1)
Reactivity
Human
(59), (33), (30), (25), (25), (13), (12), (1), (1)
Host
Mouse
(49), (11)
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
(4), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1)
Application
ELISA, Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Fluorescence Microscopy (FM)
(39), (20), (19), (10), (10), (9), (8), (4), (4), (3), (2), (1), (1)
Pubmed 2 references available
Quantity 0.1 mg
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Catalog No. ABIN1098116
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Immunogen Purified recombinant fragment of human TWF1 (AA 335-384 ) expressed in E. coli.
Clone 7C11
Isotype IgG1
Purification Purified
Alternative Name TWF1
Background Synonyms:
A6, PTK9, MGC23788, MGC41876
This gene encodes twinfilin, an actin monomer-binding protein conserved from yeast to mammals. Studies of the mouse counterpart suggest that this protein may be an actin monomer-binding protein, and its localization to cortical G-actin-rich structures may be regulated by the small GTPase RAC1.
Molecular Weight 40.3 kDa
Gene ID 5756
Application Notes Recommended Dilution:
ELISA: 1/10000, WB: 1/500 - 1/2000, IHC: 1/200 - 1/1000, ICC: 1/100, FCM: 1/200 - 1/400
Not yet tested in other applications.
Determining optimal working dilutions by titration test.
Restrictions For Research Use only
Buffer PBS with 0.05 % sodium azide and 0.5 % protein stabilizer
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Store at 4 °C or at -20 °C for long term.
Supplier Images
anti-Twinfilin, Actin-Binding Protein, Homolog 1 (Drosophila) (TWF1) (AA 335-384) antibody Red: Control Antigen (100 ng), Purple: Antigen (10ng), Green: Antigen (50 ng), Blue: Antigen (100 ng)
anti-Twinfilin, Actin-Binding Protein, Homolog 1 (Drosophila) (TWF1) (AA 335-384) antibody (2) Figure 4: Flow cytometric analysis of HeLa cells using TWF1 mouse mAb (green) and negative control (red) .
anti-Twinfilin, Actin-Binding Protein, Homolog 1 (Drosophila) (TWF1) (AA 335-384) antibody (3) Figure 3: Immunofluorescence analysis of HeLa cells using TWF1 mouse mAb (green) . Blue: DRAQ5 fluorescent DNA dye.
anti-Twinfilin, Actin-Binding Protein, Homolog 1 (Drosophila) (TWF1) (AA 335-384) antibody (4) Figure 2: Immunohistochemical analysis of paraffin-embedded ovarian cancer tissues using TWF1 mouse mAb with DAB staining.
anti-Twinfilin, Actin-Binding Protein, Homolog 1 (Drosophila) (TWF1) (AA 335-384) antibody (5) Figure 2: Immunohistochemical analysis of paraffin-embedded bladder cancer tissues using TWF1 mouse mAb with DAB staining.
anti-Twinfilin, Actin-Binding Protein, Homolog 1 (Drosophila) (TWF1) (AA 335-384) antibody (6) Figure 1: Western blot analysis using TWF1 mAb against human TWF1 recombinant protein. (Expected MW is 31.1 kDa)
anti-Twinfilin, Actin-Binding Protein, Homolog 1 (Drosophila) (TWF1) (AA 335-384) antibody (7) Figure 2: Western blot analysis using TWF1 mAb against HEK293 (1) and TWF1 (AA: 335-384) -hIgGFc transfected HEK293 (2) cell lysate.
Product cited in: Rajaraman, Brenner, Butler et al.: "Common variation in genes related to innate immunity and risk of adult glioma." in: Cancer epidemiology, biomarkers & prevention : a publication of the American Association for Cancer Research, cosponsored by the American Society of Preventive Oncology, Vol. 18, Issue 5, pp. 1651-8, 2009 (PubMed).

Rajaraman, Brenner, Neta et al.: "Risk of meningioma and common variation in genes related to innate immunity." in: Cancer epidemiology, biomarkers & prevention : a publication of the American Association for Cancer Research, cosponsored by the American Society of Preventive Oncology, Vol. 19, Issue 5, pp. 1356-61, 2010 (PubMed).

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