HAS2 antibody (AA 67-170)

Catalog No. ABIN1098124

This Mouse Monoclonal antibody specifically detects HAS2 in ELISA, WB, IHC and ICC. It exhibits reactivity toward Human and has been mentioned in 2+ publications.

Quick Overview for HAS2 antibody (AA 67-170) (ABIN1098124)

Target: HAS2 (Hyaluronan Synthase 2 (HAS2))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This HAS2 antibody is un-conjugated

Application: ELISA, Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC)

Clone: 4E7

Product Details anti-HAS2 Antibody

Binding Specificity: AA 67-170

Purpose: HAS2 Antibody

Purification: Purified antibody

Immunogen: Purified recombinant fragment of human HAS2 (AA: 67-170) expressed in E. Coli.

Isotype: IgG1

Application Details

Application Notes:

ELISA: 1/10000

ICC: 1/100 - 1/500

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Purified antibody in PBS with 0.05 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.

References for anti-HAS2 antibody (ABIN1098124)

Okuda, Kobayashi, Xia, Watabe, Pai, Hirota, Xing, Liu, Pandey, Fukuda, Modur, Ghosh, Wilber, Watabe: "Hyaluronan synthase HAS2 promotes tumor progression in bone by stimulating the interaction of breast cancer stem-like cells with macrophages and stromal cells." in: Cancer research, Vol. 72, Issue 2, pp. 537-47, (2012) (PubMed).

Jokela, Makkonen, Oikari, Kärnä, Koli, Hart, Tammi, Carlberg, Tammi: "Cellular content of UDP-N-acetylhexosamines controls hyaluronan synthase 2 expression and correlates with O-linked N-acetylglucosamine modification of transcription factors YY1 and SP1." in: The Journal of biological chemistry, Vol. 286, Issue 38, pp. 33632-40, (2011) (PubMed).

Target Details for HAS2

Target: HAS2 (Hyaluronan Synthase 2 (HAS2))

Alternative Name: HAS2

Background: Hyaluronan or hyaluronic acid (HA) is a high molecular weight unbranched polysaccharide synthesized by a wide variety of organisms from bacteria to mammals, and is a constituent of the extracellular matrix. It consists of alternating glucuronic acid and N-acetylglucosamine residues that are linked by beta-1-3 and beta-1-4 glycosidic bonds. HA is synthesized by membrane-bound synthase at the inner surface of the plasma membrane, and the chains are extruded through pore-like structures into the extracellular space. It serves a variety of functions, including space filling, lubrication of joints, and provision of a matrix through which cells can migrate. HA is actively produced during wound healing and tissue repair to provide a framework for ingrowth of blood vessels and fibroblasts. Changes in the serum concentration of HA are associated with inflammatory and degenerative arthropathies such as rheumatoid arthritis. In addition, the interaction of HA with the leukocyte receptor CD44 is important in tissue-specific homing by leukocytes, and overexpression of HA receptors has been correlated with tumor metastasis. HAS2 is a member of the newly identified vertebrate gene family encoding putative hyaluronan synthases, and its amino acid sequence shows significant homology to glycosaminoglycan synthetase (DG42) from Xenopus laevis, and human and murine hyaluronan synthase 1.

Molecular Weight: 63.5 kDa

UniProt: Q92819

Pathways: Glycosaminoglycan Metabolic Process