Toxoplasma Gondii P30 antibody

Catalog No. ABIN110947

This Mouse Monoclonal antibody specifically detects Toxoplasma Gondii P30 in WB, EIA and IF. It exhibits reactivity toward Toxoplasma gondii.

Quick Overview for Toxoplasma Gondii P30 antibody (ABIN110947)

Target: Toxoplasma Gondii P30

Reactivity: Toxoplasma gondii

Host: Mouse

Clonality: Monoclonal

Conjugate: Un-conjugated

Application: Western Blotting (WB), Enzyme Immunoassay (EIA), Immunofluorescence (IF)

Clone: P30-3

Product Details

Purification: Affinity Chromatography on Protein G

Immunogen: Native Toxoplasma gondii p30 (purified).

Isotype: IgG2a

Application Details

Application Notes: ELISA: As a guide, the end point titre in an indirect ELISA has been found to be1/2,000,000 using purified T. gondii P30 as the coating antigen. Western Blot.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: PBS buffer, pH 7.2 containing 0.09 % Sodium Azide as preservative

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details

Target: Toxoplasma Gondii P30

Background: Toxoplasma gondii p30 is the immunodominant surface antigen of Toxoplasma gondii. Toxoplasma gondii is an obligate intracellular protozoan that belongs to the class Sporozoa, and the order Eucoccidia. The parasite has an extremely wide host range and is also nonspecific with respect to the kind of cell type it can enter, making it an interesting model to study invasion. Although toxoplasmosis in healthy adults is generally asymptomatic, it produces severe disease in neonates and immunocompromised people, being the cause of death in many AIDS patients. P30 is the major surface antigen of T. gondii. It is a very abundant protein, conserved in most strains, and present both in the vesicular network of the parasitophorous vacuole and on the surface of the parasite. It is very immunogenic, eliciting high titers of antibodies in infected individuals. The gene coding for P30 has been cloned and completely sequenced: the deduced amino acid sequence shows a putative signal sequence at the N terminus, consistent with localization of P30 on the parasite's surface, as well as a short hydrophobic stretch at the C terminus.Synonyms: Major surface antigen p30

Gene ID: 5811

UniProt: P13664