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Proteasome (Prosome, Macropain) 26S Subunit, Non-ATPase, 9 (PSMD9) antibody

Antigen

Proteasome (Prosome, Macropain) 26S Subunit, Non-ATPase, 9 (PSMD9)
Synonyms p27, Rpn4, MGC8644, Bridge, Bridge-1, wu:fb30g07, zgc:92643, MGC84770, PSMD9, MGC128752, psmd9
Clonality Monoclonal (P27(26S-104))
Host
Alternatives

Mouse
Reactivity
Alternatives

Xenopus laevis
Conjugate
Alternatives Un-conjugated
Application
Alternatives Immunofluorescence (IF), Immunoprecipitation (IP), Western Blotting (WB)
3 references available
Catalog no. ABIN111067
Quantity 5 ml  (Variants)
Price 297.00 $   Plus shipping costs $45.00
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Additional Information
Application Details Publications Alternatives Variants back to top

Alternative name PSMD9
Immunogen Nuclear 26S complexes of Xenopus laevis oocytes
Cross-Reactivity Xenopus laevis
Isotype IgG1
Clone P27(26S-104)
Characteristics Synonyms: Proteasome26S
Specificity Recognizes the 20S subcomplex within the 26S hetero-oligomeric protein complex and thefree cytosolic form of 20S cylinder particles, the epitope is resistant to formaldehydefixation (up to 4%)Antigen Recognized in Species and Cultured Cell Lines (tested so far): Xenopus laevis,XLKE-A6 (Xenopus laevis), PLC (human), RVF (rat), Ptk2 (rat, kangaroo)Add. Information: * Ascites available upon request.

Application Details
Publications Alternatives Variants back to top

Application Notes Immunofluorescence microscopy: Ready to use, 20 - 30 min at RT, longer incubation mayresult in loss of soluble antigen. Western Blot: 1: 100 when using the ECL method. Immunoprecipitation (Ascites*). Affinity chromatography (Ascites*). Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Purification Supernatant
Storage Store the antibody undiluted at 2-8°C. DO NOT FREEZE! Shelf life: one year from despatch.
Restrictions For Research Use only

Publications
Application Details Alternatives Variants back to top

Peters: "Proteasomes: protein degradation machines of the cell." in: Trends in biochemical sciences, Vol. 19, Issue 9, pp. 377-82, 1995 (PubMed).

Peters, Franke, Kleinschmidt: "Distinct 19 S and 20 S subcomplexes of the 26 S proteasome and their distribution in the nucleus and the cytoplasm." in: The Journal of biological chemistry, Vol. 269, Issue 10, pp. 7709-18, 1994 (PubMed).

Peters, Cejka, Harris et al.: "Structural features of the 26 S proteasome complex." in: Journal of molecular biology, Vol. 234, Issue 4, pp. 932-7, 1994 (PubMed).

Alternatives
Application Details Publications Variants back to top

Alternatives for antigen "Proteasome (Prosome, Macropain) 26S Subunit, Non-ATPase, 9 (PSMD9)", type "Antibodies"
Hosts Rabbit (27), Mouse (2)
Reactivities Human (28), Mouse (Murine) (21), Rat (Rattus) (21), Chicken (18), Cow (Bovine) (18), Dog (Canine) (18), Guinea Pig (18), Horse (Equine) (18), Pig (Porcine) (18)
Applications Immunofluorescence (IF) (16), Western Blotting (WB) (11), ELISA (8), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (3), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (3), Immunoprecipitation (IP) (2), Immunoelectron Microscopy (IEM) (1), Immunohistochemistry (IHC) (1)
Conjugates Alexa Fluor 350 (1), Alexa Fluor 488 (1), Alexa Fluor 555 (1), Alexa Fluor 647 (1), Biotin (1), Cy3 (1), Cy5 (1), Cy5.5 (1), Cy7 (1), FITC (1), Gold (1), HRP (1), PE (1), PE,Cy3 (1), PE,Cy5 (1), PE,Cy5.5 (1), PE,Cy7 (1)
Epitopes C-Term (2)