Keratin 20 (KRT20) antibody

Details for Product No. ABIN111169
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Antigen
Synonyms KRT20, CD20, CK-20, CK20, K20, KRT21, Krt21, 9030623C06Rik
Reactivity
Human, Pig (Porcine), Rat (Rattus)
(144), (38), (36), (4), (1)
Host
Mouse
(76), (69), (2), (2)
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
(5), (4), (3), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application
Immunohistochemistry (Frozen Sections) (IHC (fro)), ELISA, Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
(104), (73), (37), (36), (25), (15), (12), (10), (4), (3), (3), (2)
Pubmed 8 references available
Quantity 50 μg
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Catalog No. ABIN111169
555.50 $
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Immunogen Electrophoretically purified Keratin K20 from human intestinal mucosa
Clone KS20-10
Isotype IgG1
Specificity IT-Ks 20.10 represents an excellent marker for certain types of carcinomas such as adenocarcinomas of the colon, transitional cell carcinomas of the bladder and Merkel cell tumors of the skin. Very sensitive detection of intestestinal and gastric foveolar epithelium, urothelial umbrella cells, Merkel cells of epidermis as well as tumors originating therefrom (e.g. primary and metastatic colorectal carcinoma). Adenocarcinomas of breast, lung, endometrium and ovary (non-mucinous) as well as neuroendocrine tumors of the lung are essentially negative. Polypeptide recognized: protein IT (keratin K20, Mr 46 000, formerly also designated cytokeratin 20). Reactivities on cultured cell lines (tested so far): HT-29, LoVo, DLD-1, SW 1116, CaCo-2, RT-4.
Purification Protein A affinity chromatography
Alternative Name Cytokeratin 20
Background Cytokeratin 20 is a type I keratin which is primarily expressed in gastric and intestinal epithelium, urothelium, and Merkel-cells. The keratins are intermediate filament proteins responsible for the structural integrity of epithelial cells and are subdivided into cytokeratins and hair keratins. The type I cytokeratins consist of acidic proteins which are arranged in pairs of heterotypic keratin chains. Their genes are clustered in a region of chromosome 17q12-q21. Cytokeratin 20 is a major cellular protein of mature enterocytes and goblet cells and is specifically expressed in the gastric and intestinal mucosa. It is also expressed in adenocarcinomas of the colon, stomach, pancreas and the bile system and is present in mucinous ovarian tumors, transitional-cell and Merkel-cell carcinomas. Notably, the squamous cell carcinomas and adenocarcinomas of the breast, lung, and endometrium, non-mucinous tumors of the ovary, and small cell carcinomas lack cytokeratin 20.
Alternate names: CK20, Cytokeratin 20, K20, KRT20, Keratin type I cytoskeletal 20, Keratin-20, Protein IT
Gene ID 54474
NCBI Accession NP_061883
UniProt P35900
Research Area Signaling, Cytoskeleton, Cytokeratins, Extracellular Matrix, Cancer
Application Notes Immunohistochemistry on frozen tissue. Immunohistochemistry on paraffin embedded sections (only after after microwavetreatment). Dilute 1/10 with PBS for immunohistochemistry. Incubation time: 1h at RT, orover night at 4C. Western blotting. ELISA. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Format Lyophilized
Reconstitution Restore with 1 mL distilled water.
Buffer Final solution contains 0.09 % Sodium azide, 0.5% BSA in PBS buffer, pH 7.4.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage -20 °C
Expiry Date 12 months
Supplier Images
anti-Keratin 20 (KRT20) antibody Keratin 20: Overview
Background publications Moll, Löwe, Laufer et al.: "Cytokeratin 20 in human carcinomas. A new histodiagnostic marker detected by monoclonal antibodies." in: The American journal of pathology, Vol. 140, Issue 2, pp. 427-47, 1992 (PubMed).

Moll, Schiller, Franke: "Identification of protein IT of the intestinal cytoskeleton as a novel type I cytokeratin with unusual properties and expression patterns." in: The Journal of cell biology, Vol. 111, Issue 2, pp. 567-80, 1990 (PubMed).

Moll, Moll: "Comparative cytokeratin analysis of sweat gland ducts and eccrine poromas." in: Archives of dermatological research, Vol. 283, Issue 5, pp. 300-9, 1991 (PubMed).

Möbus, Moll, Gerharz et al.: "Establishment of new ovarian and colon carcinoma cell lines: differentiation is only possible by cytokeratin analysis." in: British journal of cancer, Vol. 69, Issue 3, pp. 422-8, 1994 (PubMed).

Moll, Zimbelmann, Goldschmidt et al.: "The human gene encoding cytokeratin 20 and its expression during fetal development and in gastrointestinal carcinomas." in: Differentiation; research in biological diversity, Vol. 53, Issue 2, pp. 75-93, 1993 (PubMed).

Demirkesen, Hoede, Moll: "Epithelial markers and differentiation in adnexal neoplasms of the skin: an immunohistochemical study including individual cytokeratins." in: Journal of cutaneous pathology, Vol. 22, Issue 6, pp. 518-35, 1996 (PubMed).

Zhang, Oakley: "The distribution and origin of keratin 20-containing taste buds in rat and human." in: Differentiation; research in biological diversity, Vol. 61, Issue 2, pp. 121-7, 1997 (PubMed).

Harnden, Eardley, Joyce et al.: "Cytokeratin 20 as an objective marker of urothelial dysplasia." in: British journal of urology, Vol. 78, Issue 6, pp. 870-5, 1997 (PubMed).

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