The Mouse Monoclonal anti-Hepatitis A Virus antibody (Clone 818) (ABIN2859500) specifically detects Hepatitis A Virus in IF, EIA and IHC (fro).
The antibody is reactive with Hepatitis A Virus (HAV) samples.
ELISA. Immunofluorescence. Immunohistochemistry. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions
For Research Use only
Concentration
0.1 mg/mL (OD280nm, E0.1% = 1.4)
Buffer
0.01 M PBS, pH 7.2, containing 0.09 % sodium azide as preservative.
Preservative
Sodium azide
Precaution of Use
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice
Avoid repeated freezing and thawing.
Storage
-20 °C
Storage Comment
Upon receipt, aliquot and store at -20 °C.
Target
Hepatitis A Virus (HAV)
Alternative Name
Hepatitis A Virus / HAV
Target Type
Virus
Background
Hepatitis A Virus (HAV) is a 27nm nonenveloped, spherical, positive stranded RNA virus, classified within the genus hepatovirus of the picornavirus family and is among the smallest and structurally simplest of the RNA animal viruses. A single large polyprotein is expressed from a large open reading frame extending through most of the genomic RNA. This polyprotein is subsequently cleaved by a viral protease (3Cpro) to form three (possibly four) capsid proteins and several nonstructural proteins. HAV genomic replication occurs exclusively in the cytoplasm of the infected hepatocyte by a mechanism involving an RNA-dependent RNA polymerase.