Human Immunodeficiency Virus Type 1 (HIV-1) antibody (FITC)
Quick Overview for Human Immunodeficiency Virus Type 1 (HIV-1) antibody (FITC) (ABIN111704)
Target
Reactivity
Host
Clonality
Conjugate
Application
Clone
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Cross-Reactivity (Details)
- Species reactivity (tested):Human.
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Purification
- Protein A Chromatography.
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Immunogen
- Purified native HIV1 p24.
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Isotype
- IgG1
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Application Notes
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Suitable for use in IFA, Immunohistochemistry, ELISA and Western blot. Direct FA staining of target antigen in a permissive tissue culture system. Acetone fixation of the antigen source is recommended prior to staining. Not suitable for use with Paraffin Embedded Tissues.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
Restrictions
- For Research Use only
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Concentration
- 100 µg/mL (OD280 nm, E0.1% = 1.4)
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Buffer
- 0.01 M PBS, pH 7.2 containing 10 mg/mL BSA as stabilizer and 0.09 % sodium azide as preservative.
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Preservative
- Sodium azide
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Precaution of Use
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Handling Advice
- Avoid repeated freezing and thawing. This product is photosensitive and should be protected from light
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Storage
- 4 °C/-20 °C
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Storage Comment
- Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
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- Human Immunodeficiency Virus Type 1 (HIV-1)
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Alternative Name
- HIV-1
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Target Type
- Virus
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Background
- HIV1 performs highly complex orchestrated tasks during the assembly, budding, maturation and infection stages of the viral replication cycle. During viral assembly, the proteins form membrane associations and self-associations that ultimately result in budding of an immature virion from the infected cell. Gag precursors also function during viral assembly to selectively bind and package two plus strands of genomic RNA. Capsid protein p24 probably forms the conical core of the virus that encapsulates the genomic RNA-nucleocapsid complex.Synonyms: HIV-I, HIV1, Human immunodeficiency virus type 1
Target
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