Desmoglein 2 antibody

Catalog No. ABIN111817

This Mouse Monoclonal antibody specifically detects Desmoglein 2 in WB, IF, IHC (p) and IHC (fro). It exhibits reactivity toward Human, Rat, Mouse and Cow.

Quick Overview for Desmoglein 2 antibody (ABIN111817)

Target: Desmoglein 2 (DSG2)

Reactivity: Human, Rat, Mouse, Cow

Host: Mouse

Clonality: Monoclonal

Conjugate: This Desmoglein 2 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Clone: DG3-10

Product Details anti-Desmoglein 2 Antibody

Specificity: This antibody recognizes Desmoglein 1+2 (desmosome-specific cadherins) in desmosome-bearing cells. It does not react with cells containing plaque-bearing junctions of non-desmosomal type. Tumors Specifically Detected: All epithelium-derived tumors, notably carcinomas, and meningiomas. Polypeptide Reacting: Mr 165000 Desmoglein, "band 3" polypeptide of the desmosomal complex. Epitope Recognized: Localized at the repeating unit domain ("RUD") of the cytoplasmic C-terminal part of the polypeptide. Reactivities on Cultured Cell Lines (tested so far): A-431, MCF-7, TR-146, PLC and various other Human epithelial cell lines, Bovine BMGE+H, BMGE-H, MDBK.

Cross-Reactivity (Details): Species reactivity (tested):Human, Bovine, Rat and Mouse.

Purification: Affinity Chromatography on Protein A

Immunogen: "Band 3" polypeptide of isolated desmosomes from Bovine muzzle epidermis

Isotype: IgG1

Application Details

Application Notes: Western blotting. Immunofluorescence Microscopy. Immunohistochemistry on Frozen Sections. Immunohistochemistry on Paraffin Embedded Tissue (after microwave treatment). Working Dilution: Dilute at least 1/10 with PBS, pH 7.4, for Immunohistochemicalapplication. Incubation time: 1 h at RT, extended with paraffin.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Reconstitution: Restore with 1.0 mL distilled water.

Buffer: Final Solution contains PBS, pH 7.4 with 0.09 % Sodium Azide as preservative and 0.5 % BSA as stabilizer

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Prior to reconstitution store at 2-8 °C. Following reconstitution store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for Desmoglein 2

Target: Desmoglein 2 (DSG2)

Alternative Name: Desmoglein-2

Background: Desmosomes are cell-cell junctions between epithelial, myocardial and certain other cell types. Desmoglein 1 and 2 are calcium-binding transmembrane glycoprotein component of desmosomes in vertebrate epithelial cells. Currently, three desmoglein subfamily members have been identified and all are members of the cadherin cell adhesion molecule superfamily. These desmoglein gene family members are located in a cluster on chromosome 18.Synonyms: CDHF4, CDHF5, DG1, DGI, DSG-1, DSG-2, DSG1, DSG2, Desmoglein1, Desmoglein2, Desmosomal glycoprotein 1, HDGC, Pemphigus foliaceus antigen

Gene ID: 1829

NCBI Accession: NP_001934

UniProt: Q14126

Pathways: Cell-Cell Junction Organization