B-Cells (pan) antibody

Antigen: B-Cells

Epitope: pan

Clonality: Monoclonal (Ki-B1R)

Host: Mouse

Reactivity: Rat (Rattus)

Application: Immunohistochemistry (Frozen Sections) (IHC (fro)), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Catalog no.: ABIN111865

Additional Information

Characteristics: Synonyms: B Cells, B Lymphocytes, B-Lymphocytes

Alternative name: B-Cells (pan)

Immunogen: Rat LymphocytesRemarks: The epitope has not been further characterized.

Isotype: IgG1  (Matching secondary antibodies)

Clone: Ki-B1R

Description: B cells are lymphocytes that play a large role in the humoral immune response as opposedto the cell-mediated immune response that is governed by T cells. The abbreviation Bcomes from bursa of Fabricius that is an organ in birds in which avian B cells mature. Theprincipal function of B cells is to make antibodies against soluble antigens. B cells are anessential component of the adaptive immune system.

Specificity: Monoclonal Rat B-lymphocyte antibody BM4013 (clone Ki-B1R) is a pan B-cell marker usefulfor detecting B-lymphocytes and plasma cells in cell suspensions and tissue sections. Ki-B1R recognises a surface antigen of 47 kD molecular weight, which appears as a singleband under reducing conditions in SDS-PAGE. The antibody is specific for Rat mature B-lymphocytes and plasma cells. Other species nottested. Antigen Distribution: Isolated Cells: In a test comparing the immunophenotype of rat blood lymphocytes, Ki-B1Rrecognised 19% +/- 3% of peripheral blood lymphocytes (n=5). In the same test, 18% +/- 3% of the lymphocytes stained positive for sIg, whereas 79% +/-3% stained positive with Ki-T1R, the rat pan T-cell marker.

Application Details

Application Notes: Immunohistochemistry on Frozen Sections: 1 µg/ml (1/100). Immunohistochemistry on Paraffin Sections: 10 µg/ml (1/10). No antigen retrievalrequired, microwave treatment may enhance the reactivity. Suggested Positive Control: Rat spleen. Has been described to work in FACS. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.

Concentration: 0.1 mg/ml (after reconstitution)

Buffer: PBS, pH 7.2 with 10 mg/ml BSA as a stabilizer and 0.01% Thimerosal as apreservativeReconstitution: Restore with 0.5 ml distilled water.

Storage: Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing. Shelf life: one year from despatch. Pictures:

Research Area: Immunology

Restrictions: For Research Use only