B-Cells antibody

Catalog No. ABIN965633

Product Details

Target: B-Cells

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: Un-conjugated

Application: Immunohistochemistry (IHC), Western Blotting (WB)

Specificity: This antibody defines the antigen which appears on B cell progenitors following HLA-DR and preceding CD10, CD19, CD22, CD 37 and cym. It is expressed on resting B cells and then reappears and persists in the cytoplasm and on the cell surface until cytoplasmic Ig appears. It is a useful antibody for studying B cell origins. It reacts with human B cell lines Daudi, Raji, Namalva, EB-3, RPMI-8226 (50% of cells). This antibody does not label neoplasms of T cell lines, blood granulocytes, thymocytes or bone marrow stromal fibroblasts. No significant changes are detected after PHA or ConA stimulation while LPS and PWM stimulated cultures after 18-48h show decreased number of antigen-positive cells but in final terms of cultivation antigen is expressed again.

Purification: Concentrated.

Immunogen: Human B cell line Daudi.

Clone: B381 (IPO10)

Isotype: IgG3

Application Details

Application Notes: Staining Procedure: This antibody can be used on formalin-fixed, paraffin-embedded tissue sections. Prolonged fixation in buffered formalin can destroy the epitope. The antibody may be used at a dilution of 1:50-1:60 in IHC. It is recommended that this product be used on frozen tissue sections or specimens. The optimal conditions should be determined by the individual laboratory. When using paraffin-embedded and formalin-fixed tissues, high temperature antigenic unmasking technique (see instructions below) is strongly recommended for consistent and reproducible results.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.3 mg/ml.

Buffer: 20 mM tris-borate, 150 mM Sodium Chloride, dialyzed media RPMI 1640/D-MEM containing fetal serum, BMC-6 carrier polysaccharides, carrier protein, pH 7.5

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

References

Kunos: "Adrenergic receptor research: recent developments." in: Progress in drug research. Fortschritte der Arzneimittelforschung. Progrès des recherches pharmaceutiques, Vol. 33, pp. 151-67, (1990) (PubMed).

Target Details

Target: B-Cells

Alternative Name: B Cell