ITGA2 antibody

Catalog No. ABIN111931

This Mouse Monoclonal antibody specifically detects ITGA2 in IHC (fro). It exhibits reactivity toward Human.

Quick Overview for ITGA2 antibody (ABIN111931)

Target: ITGA2 (Integrin alpha 2 (ITGA2))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This ITGA2 antibody is un-conjugated

Application: Immunohistochemistry (Frozen Sections) (IHC (fro))

Clone: A-1-43

Product Details anti-ITGA2 Antibody

Specificity: The antibody A.1.43 detects CD49b and is a useful tumor progression marker, particularly also in combination with clone A.1.43 detects CD49b and is a useful tumour progression marker, particularly also in combination with clone A10-33/1 (anti gpIIb/IIIa). CD49b, integrin α2, is a transmembrane glycoprotein that is non-covalently associated with the integrin β1 chain to form the α2β1 (very late antigen (VLA) 2) complex. The α2β1 complex was originally reported on long-term activated T cells and was later shown to be identical to the gpIa/IIa complex on platelets. The α2β1 complex is a pivotal receptor for collagen, and the adhesion of platelets to collagen is known to be important for the initial step of platelet aggregation. The antibody stains an epitope on the cell surface. The antibody is positive on the following melanoma cell lines: BRO, SK-Mel-13, SK-Mel-19, SK-Mel-29, SK-Mel-113, and negative on normal monocytes. Tissue sections: Acetone fixed tissue sections of melanoma larger than 1.5 mm show positive staining whereas the majority of benign melanocytic nevi (>95 %) are negative.

Purification: Affinity chromatography.

Isotype: IgG1

Application Details

Application Notes: Suitable for Immunohistochemistry on frozen sections (0.25-2 μg/mL, 1/100-1/800) andFACS. Suggested positive control: Human skin. The antibody is positive on the following melanoma cell lines: BRO, SK-Mel-13, SK-Mel-19,SK-Mel-29, SK-Mel-113, and negative on normal monocytes.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Protocol: Protocol with frozen, ice-cold acetone-fixed sections: The whole procedure is performed at room temperature1. Wash in PBS2. Block endogenous peroxidase3. Wash in PBS4. Block with 10% normal goat serum in PBS for 30min. in a humid chamber5. Incubate with primary antibody (dilution see datasheet) for 1h in a humid chamber6. Wash in PBS7. Incubate with secondary antibody (peroxidase-conjugated goat anti mouse IgG+IgM(H+L) minimal-cross reaction to human) for 1h in a humid chamber8. Wash in PBS9. Incubate with AEC substrate (3-amino-9-ethylcarbazol) for 12min. 10. Wash in PBS11. Counterstain with Mayer's hemalum For further information and details see technicalinformation

Restrictions: For Research Use only

Handling

Reconstitution: Restore with 0.6 mL distilled water.

Concentration: 0.2 mg/mL

Buffer: PBS buffer pH 7.2 with 0.01 % Thimerosal as preservative and 1 % BSA as stabilizer.

Preservative: Thimerosal (Merthiolate)

Precaution of Use: This product contains thimerosal (merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Target Details for ITGA2

Target: ITGA2 (Integrin alpha 2 (ITGA2))

Alternative Name: CD49b / ITGA2

Background: Integrins are heterodimeric cell surface receptors composed of alpha and beta subunits, which mediate cell-cell and cell-extracellular matrix attachments. They are responsible for adhesion of platelets and other cells to collagens, modulation of collagen and collagenase gene expression, force generation and organization of newly synthesized extracellular matrix. Aberrant integrin expression has been found in many epithelial tumours. Changes in integrin expression have been shown to be important for the growth and early metastatic capacity of melanoma cells.Synonyms: CD49 antigen-like family member B, Collagen Receptor, GPIa, ITGA-2, Integrin alpha-2, Platelet membrane glycoprotein Ia, VLA-2 alpha, VLA2

Gene ID: 3673

NCBI Accession: NP_002194

UniProt: P17301

Pathways: CXCR4-mediated Signaling Events, Smooth Muscle Cell Migration, Integrin Complex