COL18A1 antibody (C-Term, Chain alpha 1)

Catalog No. ABIN111992

The Mouse Monoclonal anti-COL18A1 antibody is suitable to detect COL18A1 in samples from Human. It has been validated for IHC (p) and IHC (fro).

Quick Overview for COL18A1 antibody (C-Term, Chain alpha 1) (ABIN111992)

Target: COL18A1 (Collagen, Type XVIII, alpha 1 (COL18A1))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This COL18A1 antibody is un-conjugated

Application: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Clone: 1837-46

Product Details anti-COL18A1 Antibody

Binding Specificity: C-Term, Chain alpha 1

Specificity: This antibody is directed against the Endostatin portion (the carboxy-terminal domain) of collagen XVIII.

Purification: Affinity Chromatography

Immunogen: Recombinant Human Endostatin (the carboxy-terminal domain)

Isotype: IgG2b

Application Details

Application Notes: Immunohistochemistry on Frozen Sections: 10 μg/mL (1/100). Immunohistochemistry on Paraffin Sections: 10 μg/mL (1/100). Microwave pretreatment incitrate buffer is recommended for antigen retrieval in paraffin embedded tissues. Recommended Positive Control: Human tonsil.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Protocol: Immunohistochemistry on paraffin sections: 1) Deparaffinize slides in chloroform for 30min and rehydrate to distilled water2) Antigen retrieval: microwave treatment (700W) for 15min in 300ml 0. 01M citrate bufferpH 6. 0. 3) Incubate slides for 15min in 200ml methanol/4. 8ml 30%H2O24) Rinse slides 2x in distilled water and for 5min in TBS, pH 7. 55) Block with normal swine serum (NSS, Jackson Immunoresearch #014-000-121) diluted1: 10 in Tris buffered saline pH 7. 5, containing 0. 1% bovine serum albumin (BSA,Calbiochem). Cover sections with NSS and incubate for 15min in a humid chamber at roomtemperature (RT). 6) Remove serum from sections (do not rinse)7) Incubate with primary antibody diluted in TBS/0. 1%BSA, pH 7. 5 for 60min in a humidchamber at room temperature8) Rinse sections in TBS, pH 7. 5 for 5min, 2x9) Incubate with secondary antibody (rabbit anti mouse IgG+IgM-biotin, Dako E 0464)1: 400 diluted in TBS/0. 1%BSA, pH 7. 5 for 30min in a moist chamber, room temperature. Preincubate now StreptABComplex /HRPO in TBS/0. 1%BSA, pH 7. 5! 10) Rinse sections in TBS, pH 7. 5 for 5min, 2x11) Incubate with StreptABComplex /HRPO (DAKO, K 0377) for 30min in a humid chamber atroom temperature12) Rinse sections in TBS, pH 7. 5 for 5min, 2x13) DAB-staining Prepare 3,3 - diaminobenzidintetrahydrochloride (DAB) according themanufacturers instructions (e. g. DAB tablets) and incubate slides for 1-15 min. Check colorreaction to avoid overstaining. 14) Rinse sections in TBS, pH 7. 5 for 5min, 2x15) Counterstain in haematoxylin for 1min and rinse in cold tap water for 10min16) Mount slides with coverslips

Restrictions: For Research Use only

Handling

Reconstitution: Restore with 0.5 mL distilled water.

Concentration: 0.4 mg/mL

Buffer: Stock solution contains PBS, pH 7.2 with 5 mg/mL BSA as a stabilizer and 0.09 % Sodium Azide as preservative

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing. Should this product contain a precipitate we recommend microcentrifugation before use.

Storage: 4 °C

Storage Comment: Store the antibody undiluted at 4-8 °C for one month or at -20 °C for longer.

Target Details for COL18A1

Target: COL18A1 (Collagen, Type XVIII, alpha 1 (COL18A1))

Background: Endostatin is a powerful inhibitor of endothelial cell proliferation and angiogenesis and shows widespread distribution, mainly in vessel walls and some other basement membrane zones.Synonyms: COL18A1, Endostatin, KNO1

Gene ID: 80781

NCBI Accession: NP_085059

UniProt: P39060