Allograft Inflammatory Factor 1 (AIF1) antibody

Antigen: Allograft Inflammatory Factor 1 (AIF1)

Synonyms: IBA1, AIF-1, IRT-1, G1, Iba1, AI607846, D17H6S50E, iba1, Bart1, mrf-1, fa04b11, wu:fa04b11, AIF1, AIF, aif1

Clonality: Monoclonal (1022-5)

Host: Mouse

Reactivity: Human, Rat (Rattus)

Application: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Catalog no.: ABIN112009

Additional Information

Characteristics: Synonyms: AIF-1, Protein G1, IBA1, Allograft inflammatory factor 1, Ionized Calcium-Binding AdapterMolecule 1

Alternative name: AIF1

Swiss-Prot: P55008

Immunogen: Purified Human recombinant AIF-1. Epitope not further characterized.

Isotype: IgG2b  (Matching secondary antibodies)

Clone: 1022-5

Description: Allograft Inflammatory Factor-1 is a Ca2+-binding peptide produced by activatedmacrophages and microglial cells. It has been suggested that AIF-1 expression isassociated with chronic inflammatory processes. AIF-1, like the Ca2+-binding peptidesMRP8 and MRP14, is expressed by activated macrophages and might participate in avariety of pathogenic processes in the mammalian brain and in chronic transplantrejection. It has been shown to be expressed early and persistently in chronically rejectingcardiac allografts but not in cardiac syngrafts and host hearts. The recently describedapoptosis-inducing factor AIF is not related to AIF-1.

Specificity: The antibody reacts with AIF-1. Species Reactivity: Tested: Human and Rat.

Application Details

Protocol: Immunohistochemitry With Unlabeled Monoclonal Antibodies On Paraffin-EmbeddedTissue Sections1) Deparaffinize slides in chloroform for 30min and rehydrate to distilled water2) Antigen retrieval: microwave treatment (700W) for 15min in 300ml 0. 01M citrate bufferpH 6. 0. 3) Incubate slides for 15min in 200ml methanol/4. 8ml 30%H2O24) Rinse slides 2x in distilled water and for 5min in TBS, pH 7. 55) Block with normal swine serum diluted 1: 10 in Tris buffered saline pH 7. 5, containing0. 1% bovine serum albumin (BSA). Cover sections with NSS and incubate for 15min in ahumid chamber at room temperature (RT). 6) Remove serum from sections (do not rinse)7) Incubate with primary antibody diluted in TBS/0. 1%BSA, pH 7. 5 for 60min in a humidchamber at room temperature8) Rinse sections in TBS, pH 7. 5 for 5min, 2x9) Incubate with secondary antibody (rabbit anti mouse IgG+IgM-biotin) 1: 400 diluted inTBS/0. 1%BSA, pH 7. 5 for 30min in a moist chamber, room temperature. Preincubate nowStreptABComplex /HRPO in TBS/0. 1%BSA, pH 7. 5! 10) Rinse sections in TBS, pH 7. 5 for 5min, 2x11) Incubate with StreptABComplex /HRPO for 30min in a humid chamber at roomtemperature12) Rinse sections in TBS, pH 7. 5 for 5min, 2x13) DAB-staining Prepare 3,3' - diaminobenzidintetrahydrochloride (DAB) according themanufacturers instructions (e. g. DAB tablets) and incubate slides for 1-15 min. Check colorreaction to avoid overstaining. 14) Rinse sections in TBS, pH 7. 5 for 5min, 2x15) Counterstain in haematoxylin for 1min and rinse in cold tap water for 10min16) Mount slides with coverslips

Application Notes: Immunohistochemistry on Paraffin Sctions: 10-20 μg/ml (1/20-1/0). Mcrowavepretreatment for antigen retrieval recommended. Suggested positive control: Human spleen. Does not react on routinely processed Frozen Sections. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.

Concentration: 0.4 mg/ml

Buffer: PBS, pH 7.2, containing 5 mg/ml BSA as stabilizer and 0.01% Kathon aspreservative.Reconstitution: Restore by adding 0.5 ml distilled water.

Storage: Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing. Shelf life: one year from despatch.

Research Area: Inflammation

Restrictions: For Research Use only