Allograft Inflammatory Factor 1 (AIF1) antibody (Biotin)

Antigen: Allograft Inflammatory Factor 1 (AIF1)

Synonyms: IBA1, AIF-1, IRT-1, G1, Iba1, AI607846, D17H6S50E, iba1, Bart1, mrf-1, fa04b11, wu:fa04b11, AIF1, AIF, aif1

Clonality: Monoclonal (1022-5)

Host: Mouse

Reactivity: Human, Rat (Rattus)

Conjugate: Biotin

Application: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Catalog no.: ABIN112010

Additional Information

Characteristics: Synonyms: AIF-1, Protein G1, IBA1, Allograft inflammatory factor 1, Ionized Calcium-Binding AdapterMolecule 1

Alternative name: AIF1

Swiss-Prot: P55008

Immunogen: Purified Human recombinant AIF-1Epitope is not further characterized.

Isotype: IgG2b

Clone: 1022-5

Description: Allograft Inflammatory Factor-1 is a Ca2+-binding peptide produced by activatedmacrophages and microglial cells. It has been suggested that AIF-1 expression isassociatedwith chronic inflammatory processes. AIF-1, like the Ca2+-binding peptides MRP8 andMRP14, is expressed by activated macrophages and might participate in a variety ofpathogenic processes in the mammalian brain and in chronic transplant rejection. It hasbeen shown to be expressed early and persistently in chronically rejecting cardiacallografts but not in cardiac syngrafts and host hearts. The recently describedapoptosis-inducing factor AIF is not related to AIF-1.

Specificity: Recognizes AIF-1. This antibody ABIN112010 reacts with activated Macrophages / Microglial cells. Species: Human and Rat. Others not tested.

Application Details

Application Notes: Immunohistochemistry on Paraffin Sections: 5 µg/ml (1/40). Pretreatment is not necessary. Suggested Positive Control: Human spleen. This antibody does not react on routinely processed Frozen Sections. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.

Concentration: 0.2 mg/ml

Buffer: PBS containing 5 mg/ml BSA as a stabilizer and 0.09% Sodium Azide as apreservative.Label: BiotinReconstitution: Restore by adding 0.5 ml distilled water.

Storage: Store the original vial at 2-8°C, stock solution or aliquots thereof at -20°C. Avoid repeated thawing and freezing. Shelf life: one year from despatch.

Research Area: Inflammation

Restrictions: For Research Use only

Publications

Publications: Schluesener, Seid, Meyermann: "Effects of autoantigen and dexamethasone treatment on expression of endothelial-monocyte activating polypeptide II and allograft-inflammatory factor-1 by activated macrophages and microglial cells in lesions of experimental autoimmune encephalomyelitis, n" in: Acta neuropathologica, Vol. 97, Issue 2, pp. 119-26, 1999 (PubMed).