Low Density Lipoprotein Receptor-Related Protein Associated Protein 1 (LRPAP1) antibody

Antigen: Low Density Lipoprotein Receptor-Related Protein Associated Protein 1 (LRPAP1)

Synonyms: RAP, MRAP, A2RAP, HBP44, A2MRAP, MGC138272, C77774, AA617339, AI790446, AU042172, LRPAP1, wu:fi20f07, wu:fi22c06, MGC139402, DKFZp459I2430

Clonality: Monoclonal (7F1)

Host: Mouse

Reactivity: Human

Conjugate: Un-conjugated

Application: Immunofluorescence (IF), Western Blotting (WB), Immunohistochemistry (IHC)

Catalog no.: ABIN112189

Additional Information

Alternative name: LRPAP1 / A2MRAP

UniProt: P30533

Immunogen: Human RAP.

Cross-Reactivity: Human

Isotype: IgG1

Clone: 7F1

Description: Low density lipoprotein receptor-related protein-associated protein 1 (RAP) interacts withLRP1/alpha-2-macroglobulin receptor and glycoprotein 330. It is present on the cell surfaceforming a complex with the alpha 2 macroglobulin receptor heavy and light chains. BindsLRP1B, binding is followed by internalization and degradation. In complex with the alpha 2MR or gp330, it may have some role in the pathogenesis of membrane glomerularnephritis.

Characteristics: Synonyms: Alpha-2-MRAP, RAP, Alpha-2-macroglobulin receptor-associated protein, Low densitylipoprotein receptor-related protein-associated protein 1

Specificity: This antibody BM5054 (7F1) reacts with the 39 kDa RAP protein, which is cosynthesizedwith Human LRP/alpha2MR (low density lipoprotein receptor-relatedprotein/alpha2-Macro-Globulin Receptor). It inhibits the binding of ligands, e. g. tPA, uPA, VLDL, liproprotein lipase, andalpha2-Macroglobulin-proteinase complexes to LRP/A2MR. Species: Human. Others not tested.

Application Details

Application Notes: Immunoblotting. Immunofluorescence. Immunohistochemistry: 1/20. Ligand-binding assays. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.

Purification: Purified

Buffer: PBS buffer, pH 7.4 containing 0.5% BSA as stabilizer.Reconstitution: Restore in 1 ml distilled water.

Storage: Prior to reconstitution store at 2-8°C. Following reconstitution store the antibody at -20°C. Avoid repeated freezing and thawing. Shelf life: one year from despatch.

Restrictions: For Research Use only

Publications

Kounnas, Argraves, Strickland: "The 39-kDa receptor-associated protein interacts with two members of the low density lipoprotein receptor family, alpha 2-macroglobulin receptor and glycoprotein 330." in: The Journal of biological chemistry, Vol. 267, Issue 29, pp. 21162-6, 1992 (PubMed).