Actin, Alpha, Cardiac Muscle 1 (ACTC1) antibody

Antigen: Actin, Alpha, Cardiac Muscle 1 (ACTC1)

Synonyms: ACTC, ASD5, CMD1R, CMH11, MGC156490, actcl, actc1l, ACTC1, actc, cmd1r, MGC75679, ACTBL2, MGC128362, ACTA, ASMA, CFTD, MPFD, NEM1, NEM2, NEM3, CFTD1, CFTDM, Acts, Acta-2, Actsk-1, AA959943, fa27h01, fb83f06, wu:fa27h01, wu:fb83f06, ACTA1, acta1, MGC52636, MGC79039, ACTB, acta, asma, cftd, mpfd, nem1, nem2, nem3, acta2, cftd1, cftdm, MGC89192, ACTG1, LOC100154254, DKFZp468G072, Actc-1

Clonality: Monoclonal (AC1-20-4-2)

Host: Mouse

Reactivity: Cow (Bovine)

Application: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Catalog no.: ABIN112213

Additional Information

Alternative name: Alpha 1 cardiac muscle Actin / ACTC1

UniProt: P68032

Immunogen: Synthetic NH2 terminus decapeptide of cardiac isoform of Actin.

Cross-Reactivity: Cow (Bovine), Chicken, Human, Rabbit

Isotype: IgG1

Clone: AC1-20-4-2

Description: Actins are highly conserved proteins that are involved in cell motility, structure, andintegrity. ACTB/ACTC are nonmuscle cytoskeletal actins and major constituents of thecontractile apparatus. Defects in ACTB are a cause of juvenile-onset dystonia. Defects inACTC have been associated with idiopathic dilated cardiomyopathy (IDC) and familialhypertrophic cardiomyopathy (FHC). Fetal Actin can be localized in regenerating skeletal muscle after injury (in satellite cells)and in veins of the umbelical cord.

Specificity: This antibody is specific for Fetal (Cardiac) isoform of Actin. Clone Ac1 represents an excellent marker for cardiac tissue. It discriminates fetal (cardiac)alpha-Actin from all other Actin isoforms. Mab Ac1-20. 4. 2 shows no cross reaction with other Actin isoforms present in skeletal andsmooth muscle, provided that stringent conditions have been applied (see SpecialTreatment). Species Reactivity: Tested: Human, Bovine, Rabbit and Chicken.

Synonyms: ACTC, ASD5, CMD1R, CMH11, MGC156490, actcl, actc1l, ACTC1, actc, cmd1r, MGC75679, ACTBL2, MGC128362, ACTA, ASMA, CFTD, MPFD, NEM1, NEM2, NEM3, CFTD1, CFTDM, Acts, Acta-2, Actsk-1, AA959943, fa27h01, fb83f06, wu:fa27h01, wu:fb83f06, ACTA1, acta1, MGC52636, MGC79039, ACTB, acta, asma, cftd, mpfd, nem1, nem2, nem3, acta2, cftd1, cftdm, MGC89192, ACTG1, LOC100154254, DKFZp468G072, Actc-1

Application Details

Application Notes: ELISA: 1/500. Western Blot: 1/1000 (using the ECL-enhanced procedure). Immunohistochemistry on Frozen Sections. Immunohistochemistry on Paraffin-Embedded Tissue: 1/10 (After microwave treatment). Incubation time: 1 h at RT. Special Treatment: It is necessary to include 0.5 M NaCl in all washing buffers forapplication on Frozen tissue and 1 M NaCl for Western blotting and ELISA to enhancespecifity. This is not necessary for Paraffin sections. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.

Purification: Purified

Buffer: Final Solution contains PBS, pH 7.4 with 0.09% Sodium Azide aspreservative and 0.5% BSA as stabilizer.Reconstitution: Restore in 1.0 ml distilled water.

Storage: Store the antibody After reconstitution at 2-8°C. Shelf life: one year from despatch.

Research Area: Extracellular Matrix

Restrictions: For Research Use only

Publications

Franke, Stehr, Stumpp et al.: "Specific immunohistochemical detection of cardiac/fetal alpha-actin in human cardiomyocytes and regenerating skeletal muscle cells." in: Differentiation; research in biological diversity, Vol. 60, Issue 4, pp. 245-50, 1996 (PubMed).

Rudy, Yatskievych, Antin et al.: "Assembly of thick, thin, and titin filaments in chick precardiac explants." in: Developmental dynamics : an official publication of the American Association of Anatomists, Vol. 221, Issue 1, pp. 61-71, 2001 (PubMed).