ACTC1 antibody (N-Term)

Catalog No. ABIN224333

There is 1 publication for this product available. The Mouse Monoclonal anti-ACTC1 antibody is suitable to detect ACTC1 in samples from Human, Rabbit, Cow and Chicken. It has been validated for WB, IHC, ELISA, IHC (p) and IHC (fro).

Quick Overview for ACTC1 antibody (N-Term) (ABIN224333)

Target: ACTC1 (Actin, Alpha, Cardiac Muscle 1 (ACTC1))

Reactivity: Human, Rabbit, Cow, Chicken

Host: Mouse

Clonality: Monoclonal

Conjugate: This ACTC1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Product Details anti-ACTC1 Antibody

Binding Specificity: N-Term

Specificity: Recognizes fetal cardiac actin. Represents an excellent marker for cardiac tissue, it discriminates fetal (cardiac) a-actin from all other actin isoforms. Shows no cross-reaction with other actin isoforms present in skeletal and smooth muscle, provided that stringent experimental conditions have been applied. Species cross-reactivity: Human, bovine, rabbit, chicken.

Purification: Protein G purified

Immunogen: Synthetic N-terminus decapeptide of cardiac isoform of actin.

Type of Immunogen: Synthetic peptide

Isotype: IgG1

Application Details

Application Notes: Approved: ELISA (1:500), IHC, IHC-Fr, IHC-P, WB (1:1000)

Usage: Suitable for use in Western Blot, ELISA and Immunohistochemistry. Immunohistochemistry: 1:10. Frozen and paraffin sections. Note: It is necessary to include 0.5 M sodium chloride in all washing buffers for application on frozen tissue. This is not necessary for paraffin sections. Western Blot: 1:1000 (using the ECL-enhanced procedure). ELISA: 1:500. Note: It is necessary to include 1 M sodium chloride for application in Western blot and ELISA to enhance specificity.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Sterile ddH2O

Concentration: Lot specific

Buffer: Lyophilized powder from PBS,  pH 7.4, 0.5 % BSA, 0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Aliquot to Avoid repeated freezing and thawing

Storage: -20 °C

Storage Comment: Reconstituted or lyophilized: store at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid freeze-thaw cycles.

References for anti-ACTC1 antibody (ABIN224333)

Itier, Ret, Viale, Sweet, Bangari, Caron, Le-Gall, Bénichou, Leonard, Deleuze, Orsini: "Effective clearance of GL-3 in a human iPSC-derived cardiomyocyte model of Fabry disease." in: Journal of inherited metabolic disease, (2014) (PubMed).

Target Details for ACTC1

Target: ACTC1 (Actin, Alpha, Cardiac Muscle 1 (ACTC1))

Alternative Name: ACTC1 / Alpha Cardiac Actin

Background: Name/Gene ID: ACTC1

Synonyms: ACTC1, Actin, alpha cardiac muscle 1, Actin, alpha, cardiac muscle, Actin, alpha, cardiac muscle 1, Alpha-cardiac actin, CMH11, CMD1R, ACTC, LVNC4, ASD5

Gene ID: 70

UniProt: P68032

Pathways: Myometrial Relaxation and Contraction