Desmocollin 1 antibody

Catalog No. ABIN112238

The Mouse Monoclonal anti-Desmocollin 1 antibody is suitable to detect Desmocollin 1 in samples from Human. It has been validated for WB, IHC (p) and IHC (fro).

Quick Overview for Desmocollin 1 antibody (ABIN112238)

Target: Desmocollin 1 (DSC1)

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This Desmocollin 1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Clone: Dsc-1-U100

Product Details anti-Desmocollin 1 Antibody

Sequence: HQTLESVKGV GQGDTGRYAT DWQC

Specificity: Reacts with 111 kDa (form "a") and 103 kDa (form "b") polypeptides (Desmocollin 1 splice forms, both at pI 5.2) from Human epidermal desmosomes. Mab localizes Dsc1 present in - suprabasal layers of interfollicular epidermis - specific cell layers (e.g. in hair follicle root sheath) - Hassall bodies (spheroidal formations of densely packed reticulum-derived cells in the thymus).

Purification: Affinity Chromatography on Protein A

Immunogen: Synthetic peptide corresponding to sequence present within the intracellular part of Human Desmocollin 1

Isotype: IgG1

Application Details

Application Notes: Imunoblotting. Immunohistochemistry on frozen sections: 1: 10, 1 h at RT or overnight at 2-8C,preincubation with 0.05 - 0.2 % Triton X-100, for 5-10 min, depending on tissue type, isrecommended, Without detergent pretreatment a cytoplasmic component mightcross-react (e. g. in fibroblasts). Immunohistochemistry on paraffin-embedded sections (after microwave treatment): 1: 10, 1h at RT or overnight at 2-8C, a slight crossreactivity with a lymphocyte subpopulation isabolished by mild trypsinization after the microwave step)
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Reconstitution: Restore in 1 mL dist. water.

Buffer: PBS buffer, pH 7.4 containing 0.09 % Sodium Azide as preservative and 0.5 % BSA as stabilizer

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Prior to reconstitution store at 2-8 °C. Following reconstitution store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for Desmocollin 1

Target: Desmocollin 1 (DSC1)

Alternative Name: Desmocollin-1

Background: Desmocollin 1 is a component of intercellular desmosome junctions. It is involved in the interaction of plaque proteins and intermediate filaments mediating cell-cell adhesion. It may contribute to epidermal cell positioning (stratification) by mediating differential adhesiveness between cells that express different isoforms. Desmocollin 1 is linked to the keratinization of epithelial tissues.Synonyms: CDHF1, Cadherin family member 1, DG2/DG3, DSC1, Desmosomal glycoprotein 2/3

Gene ID: 1823

NCBI Accession: NP_004939

UniProt: Q08554