Keratin 4/5/6/8/10/13/18 (KRT4/5/6/8/10/13/18) antibody

Antigen: Keratin 4/5/6/8/10/13/18 (KRT4/5/6/8/10/13/18)

Clonality: Monoclonal (C-11)

Host: Mouse

Application: Flow Cytometry (FACS), Immunofluorescence (IF), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)

Catalog no.: ABIN112315

Additional Information

Characteristics: Synonyms: pan Keratin, Cytokeratin Pan-reactive

Alternative name: Cytokeratin (pan) (4+5+6+8+10+13+18)

Immunogen: Keratin-enriched preparation from human epidermoid carcinoma cell line A431

Isotype: IgG1  (Matching secondary antibodies)

Clone: C-11

Description: Cytokeratins are a subfamily of intermediate filaments and characterized by remarkablebiochemical diversity. Cytokeratins are represented in epithelial tissues by at least 20different polypeptides, molecular weight between 40 kDa and 68 kDa. The individualcytokeratin polypeptides are designated 1 to 20 and divided into the type I (acidiccytokeratins 9-20) and type II (basic to neutral cytokeratins 1-8) families.

Specificity: The antibody reacts with Cytokeratin peptides 4, 5, 6, 8, 10, 13, 18 in all species(recognized epitope conserved within all species). Cytokeratins are a member ofintermediate filaments subfamily represented in epithelial tissues. Species: Mammalian. Others not tested.

Application Details

Application Notes: Western Blotting. Flow Cytometry. Immunoprecipitation. Immunocytochemistry. Immunohistochemistry (Paraffin Sections). Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.

Concentration: 1.0 mg/ml

Buffer: PBS, pH~7.4 with 15 mM Sodium Azide as preservative.

Storage: Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing. Shelf life: one year from despatch.

Restrictions: For Research Use only

Publications

Publications: Bártek, Vojtĕsek, Stasková et al.: "A series of 14 new monoclonal antibodies to keratins: characterization and value in diagnostic histopathology." in: The Journal of pathology, Vol. 164, Issue 3, pp. 215-24, 1991 (PubMed).