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anti-Cytokeratin 3+2P antibody

Overview

Antigen: Cytokeratin 3+2P
Clonality: Monoclonal (AE5)
Application: Immunohistochemistry (Frozen Sections) (IHC (fro)), Western Blotting (WB)
Reactivity: Cow (Bovine), Human, Rabbit
Host: Mouse
1 reference available 1 reference available
Quantity: 0.2 mg (1.0 mg/ml)
Price: Product not available in this region.
Order number: ABIN112318
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Additional Information  » anti-Cytokeratin 3+2P antibody

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Antigen Cytokeratin 3+2P
Immunogen Human epithelial keratin
Reactivity Cow (Bovine), Human, Rabbit
Clonality Monoclonal
Format Purified
Isotype IgG1 »Matching secondary antibodies
Description Alternate Names: KRT-12, KRT12, Cytokeratin12, Cytokeratin-12, Keratin-12, Keratin12, SwissProtID: Q99456, Keratin type I cytoskeletal 12, Keratin type II cytoskeletal 2 oral.
Clone AE5
Host Mouse
Specificity AE 5 represents an excellent marker for corneal type differentiation. Positive for epithelial cells of cornea, snout and some oral mucosa. This antibody has been used for studying corneal epithelial stem cells. Mr 64 000 polypeptide (keratin K3, formerly also designated cytokeratin 3) of human corneal epithelium and keratin K76 (formerly also designated cytokeratin K2p) of palate epithelium. In cow and rabbit it reacts with lip and snout epithelia.

Application Details  » anti-Cytokeratin 3+2P antibody

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Application Immunohistochemistry (Frozen Sections) (IHC (fro)), Western Blotting (WB)
» Click here to view 3 available protocols!
Application Notes Immunohistochemistry on Frozen Sections: 1/50 1h at RT. Immunoblotting (1/500). Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Concentration 1.0 mg/ml
Purification Ion Exchange Chromatography
Buffer PBS, pH 7,2 containing 0.09% Sodium Azide as preservative.
Storage Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing. Shelf Life: one year from despatch.
Research Area Extracellular Matrix
Restrictions For Research Use only

Publications  » anti-Cytokeratin 3+2P antibody

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Publications Cooper, Schermer, Sun: "Classification of human epithelia and their neoplasms using monoclonal antibodies to keratins: strategies, applications, and limitations." In: Laboratory investigation; a journal of technical methods and pathology , Vol. 52, Issue 3, pp. 243-56, 1985 (PubMed)