GNRHR antibody (Extracellular Domain, N-Term)

Catalog No. ABIN112338

The Mouse Monoclonal anti-GNRHR antibody (Clone A9E4) (ABIN112338) specifically detects GNRHR in WB, FACS, IHC (fro) and EIA. The antibody is reactive with Human samples.

Quick Overview for GNRHR antibody (Extracellular Domain, N-Term) (ABIN112338)

Target: GNRHR (Gonadotropin-Releasing Hormone Receptor (GNRHR))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This GNRHR antibody is un-conjugated

Application: Western Blotting (WB), Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)), Enzyme Immunoassay (EIA)

Clone: A9E4

Product Details anti-GNRHR Antibody

Binding Specificity: AA 1-29, Extracellular Domain, N-Term

Specificity: This Monoclonal antibody A9E4 reacts with GnRH receptors in the anterior pituitary.

Purification: Affinity Chromatography on Protein A

Immunogen: Synthetic peptide corresponding to the amino acids 1-29 of the Human GnRH receptor extracellular domain conjugated to BSA.

Isotype: IgG1

Application Details

Application Notes: Immunohistochemistry on Frozen Tissues. (Conditions described by customer: use antibody at 8 μg/mL for 60 min at RT. Staining offormalin-fixed tissues requires boiling tissue sections in 1 mM EDTA, pH 8.0, for 10-20 min. ,followed by cooling at RT for 20 min. ). Western Blot. ELISA. Flow Cytometry.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: PBS, 0.09 % Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for GNRHR

Target: GNRHR (Gonadotropin-Releasing Hormone Receptor (GNRHR))

Alternative Name: GnRH Receptor / GNRHR

Background: Gonadotropin releasing hormone (GnRH) receptor is a protein of 54-60 kDa. GnRH receptor contains seven hydrophobic transmembrane domains connected by hydrophilic extracellular and intracellular loops characteristic of G-protein coupled receptors. GnRH stimulates the gonadotrophs of the anterior pituitary to secrete luteinizing hormone (LH) as well as follicle-stimulated hormone (FSH). The receptor contains of seven hydrophobic transmembrane domains connected by hydrophillic extracellular, and intracellular loops characteristic of G-protein coupled receptors.Synonyms: GRHR, GnRH-R, Gonadotropin-releasing hormone receptor

Gene ID: 2798

NCBI Accession: NP_000397

UniProt: P30968