CTNNA3 antibody (N-Term)

Catalog No. ABIN112367

The Mouse Monoclonal anti-CTNNA3 antibody is suitable to detect CTNNA3 in samples from Human. It has been validated for WB, IP, IF, IHC (p), IHC (fro) and FACS.

Quick Overview for CTNNA3 antibody (N-Term) (ABIN112367)

Target: CTNNA3 (Catenin (Cadherin-Associated Protein), alpha 3 (CTNNA3))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CTNNA3 antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro)), Flow Cytometry (FACS)

Clone: 892_24D2S

Product Details anti-CTNNA3 Antibody

Binding Specificity: AA 164-177, N-Term

Specificity: The 892_24D2S antibody against αT-Catenin does not show any cross reaction with αE-Catenin or αN-Catenin in western blot analysis. This 892_24D2S antibody reacts with an epitope located between residues 164-177 of the Human αT-Catenin protein.

Purification: Purified

Immunogen: Synthetic peptide corresponding to amino acids 164-177 of the human alphaT-catenin protein including an additional N-terminal cysteine (CVANKSDLQKTYQKL) coupled to keyhole limpet hemocyanin.

Isotype: IgG2a

Application Details

Application Notes: Immunofluorescence: aceton-fixed cells. Immunocytochemistry: methanol and paraformaldehyde-fixed cells, followed bypermeabilisation in 0.2 % Triton-X 100 for 2 min. Immunohistochemistry1: 1/25-1/200 with avidin-biotinylated horseradish peroxidasecomplex (ABC) as detection reagent,Immunoprecipitation.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: PBS with 0.09 % Sodium Azide as preservative

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store lyophilized at 2-8 °C and reconstituted (in aliquots) at -20 °C.

Target Details for CTNNA3

Target: CTNNA3 (Catenin (Cadherin-Associated Protein), alpha 3 (CTNNA3))

Alternative Name: Catenin alpha-3 / CTNNA3

Background: Catenins are proteins that connect cadherin/β- catenin cell adhesion complexes to the actin cytoskeleton. Although originally discovered in testis, αT-catenin is expressed in other tissues, the highest levels being observed in heart. Immunohistochemical analysis showed human αTcatenin localization at intercalated discs of cardiomyocytes and in peritubular myoid cells of testis. It is proven that αT-catenin can functionally restore cell-cell adhesion in colon cancer cells lacking α-catenins. This indicates that this protein is involved in the formation of specific cell-cell contacts in specific types of muscle cells. The encoded protein has about the same predicted size (100 kDa) as other α-catenins to which it shows an overall amino acid identity of 57 % . In cells transfected with αT-catenin cDNA, interaction with β-catenin was demonstrated by coimmunoprecipitation.Synonyms: Alpha Catenin 3, Alpha T-catenin, Cadherin-associated protein

Gene ID: 29119

NCBI Accession: NP_001120856

UniProt: Q9UI47