The FITC-conjugated Rabbit Polyclonal anti-Legionella Pneumophila antibody (ABIN112941) specifically detects Legionella Pneumophila in IF.
The antibody is reactive with Legionella pneumophila samples.
Fluorescence microscopy (Titer: >1: 800 by indirect IFA vs. acetone-fixed organisms). Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions
For Research Use only
Format
Liquid
Concentration
4-5 mg/mL (OD280 nm, E0.1%=1.4)
Buffer
0.01 M PBS pH 7.2 with 10 mg/mL BSA and 0.09 % sodium azide as preservative.
Preservative
Sodium azide
Precaution of Use
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice
Avoid repeated freezing and thawing.
Storage
4 °C/-20 °C
Storage Comment
Store the antibody at 2-8 °C for one month or (in aliquots) at -20 °C for longer. Do not freeze working dilutions
Target
Legionella Pneumophila
Target Type
Bacteria
Background
Legionella pneumophila is a flagellated gram negative bacterium found primarily in warm water environments. Legionella pneumophila causes a type of pneumonia called Legionnaire disease and a milder condition called Pontiac fever. Infection is acquired through inhalation of the microorganism in water mists (e.g. from air conditioning, cooling towers, whirlpool spas and showers mists). A number of risk factors for acquiring Legionnaire disease have been identified, including age, smoking, chronic lung disease, cancer, and immunosuppression.