Topoisomerase I antibody

Catalog No. ABIN112981

This Human Polyclonal antibody specifically detects Topoisomerase I in WB, ID and EIA. It exhibits reactivity toward Human.

Quick Overview for Topoisomerase I antibody (ABIN112981)

Target: Topoisomerase I (TOP1) (Topoisomerase (DNA) I (TOP1))

Reactivity: Human

Host: Human

Clonality: Polyclonal

Conjugate: This Topoisomerase I antibody is un-conjugated

Application: Western Blotting (WB), Immunodiffusion (ID), Enzyme Immunoassay (EIA)

Product Details anti-Topoisomerase I Antibody

Specificity: Shows only the Scl-70 precipitate band when tested using double immunodiffusion against rabbit thymus extract. Specificity is verified by identity of precipitation with CDC-AF reference serum. The titer obtained will vary with the test system, however, undiluted antibody shows a strong Scl-70 precipitate band. Product shows no reactivity at a dilution of 1:100 when tested against purified SSA, SSB, Sm, Sm/RNp and Jo-1 antigen in an ELISA system designed to detect all IgG subclasses.

Purification: Precipitation of Ig fraction from delipidized and defibrinated human plasma.

Application Details

Application Notes: Suitable for use in Immunodiffusion and ELISA.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Format: Liquid

Reconstitution: Reconstitute with 2 mL deionized water.

Buffer: 0.01 M PBS, 0.13 M NaCl containing 0.02 % Sodium azide as preservative.

Preservative: Sodium azide

Precaution of Use: Do Not Add Sodium Azide.

Handling Advice: Avoid repeated freezing and thawing. Dilute only prior to immediate use.

Storage: 4 °C/-20 °C

Storage Comment: Store vial at 2-8 °C prior to restoration. Restore with deionized water (or equivalent), centrifuge product if not completely clear after standing at room temperature. This product is stable for one month at 2-8 °C as an undiluted liquid. For extended storage reconstitute product with 50% glycerol instead of water and then aliquot contents and freeze at -20 °C or below.

Target Details for Topoisomerase I

Target: Topoisomerase I (TOP1) (Topoisomerase (DNA) I (TOP1))

Background: Synonyms: DNA topoisomerase 1, DNA topoisomerase I, Top-1

Gene ID: 7150

NCBI Accession: NP_003277

UniProt: P11387

Pathways: Caspase Cascade in Apoptosis, Stem Cell Maintenance