Lipopolysaccharides (LPS) antibody

Catalog No. ABIN113127

The Goat Polyclonal anti-Lipopolysaccharides (LPS) antibody (ABIN113127) specifically detects Lipopolysaccharides (LPS) in IF. The antibody is reactive with Gram Negative Bacteria samples.

Quick Overview for Lipopolysaccharides (LPS) antibody (ABIN113127)

Target: Lipopolysaccharides (LPS)

Reactivity: Gram Negative Bacteria

Host: Goat

Clonality: Polyclonal

Conjugate: This Lipopolysaccharides (LPS) antibody is un-conjugated

Application: Immunofluorescence (IF)

Product Details anti-Lipopolysaccharides (LPS) Antibody

Purification: Sodium Sulphate Precipitation and Ion Exchange Chromatography

Immunogen: Whole cells prep of Lipid A from E. coli O157

Application Details

Application Notes: Indirect Immunofluoresence: > 1/100 (against members of the Entero-bacteriaciae). May also be used in place of neat antiserum in almost any appropriate antibody-basedtechnique. Also suitable for conjugation purposes.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 4-5 mg/mL (OD280 nm, E0.1% = 1.4)

Buffer: 0.01 M PBS, pH 7.2, 0.09 % Sodium Azide, None

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for Lipopolysaccharides (LPS)

Target: Lipopolysaccharides (LPS)

Target Type: Chemical

Background: Lipid A is a lipid component of an endotoxin held responsible for toxicity of Gram-negative bacteria. Sensing of lipid A by the human immune system may also be critical for the onset of immune responses to Gram-negative infection, and for the subsequent successful fight against the infection. Lipid A is located at one end of the lipopolysaccharide (LPS, also called endotoxin) molecule, and anchors the LPS to the outer membrane of a Gram-negative bacteria. Many of the immune activating abilities of LPS can be contributed to the lipid A unit. It is a very potent stimulant of the immune system, activating cells (for example, monocytes or macrophages) at picogram per milliliter quantities. When present in the body at high concentrations during a Gram-negative bacterial infection, it may cause shock and death by an "out of control" excessive immune reaction.Synonyms: LPS, Lipid A