LDLR antibody (Extracellular Domain)

Catalog No. ABIN113461

This Rabbit Polyclonal antibody specifically detects LDLR in WB, FACS and IHC (fro). It exhibits reactivity toward Humanand has been mentioned in 1 publication.

Quick Overview for LDLR antibody (Extracellular Domain) (ABIN113461)

Target: LDLR (Low Density Lipoprotein Receptor (LDLR))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This LDLR antibody is un-conjugated

Application: Western Blotting (WB), Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro))

Product Details anti-LDLR Antibody

Binding Specificity: AA 184-195, Extracellular Domain

Specificity: The antibody reacts specifically with The LDL Receptor (160 kDa mature or glycosylated receptor, 120 kDa precursor or unglycosylated receptor) plays a key role in cellular cholesterol homeostasis. It does not inhibit binding of LDL.

Cross-Reactivity (Details): Species reactivity (tested):Human.

Purification: Immunoaffinity Chromatography

Immunogen: Specific synthetic peptide (sequence not conserved in VLDL receptor and LRP) of the LDL receptor extracellular domain (epitope between residues 184-195, the linker region between repeats 4 and 5)

Application Details

Application Notes: Flow Cytometry: 1/10. Immunobloting: 1/100-1/200. Recognizes the 160 kDa band of LDLR and a 120 kDa band ofthe LDLR precursor from Fibroblasts, Hepatocytes, and monocytic cells cultured in thepresence of lipoprotein-deficient serum. Immunohistochemistry: 1/20. Receptor Binding Studies.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.1 mg/mL

Buffer: PBS, 0.5 % BSA

Handling Advice: Avoid repeated freezing and thawing.

Storage: -20 °C

Storage Comment: Store the lyophilized antibody at -20 °C. Following reconstitution, the product ist stable at 2-8 °C for up one week or (in aliquots) at -20 °C for up to six months.

References for anti-LDLR antibody (ABIN113461)

Akazawa, Date, Morikawa, Murayama, Miyamoto, Kaga, Barth, Baumert, Dubuisson, Wakita: "CD81 expression is important for the permissiveness of Huh7 cell clones for heterogeneous hepatitis C virus infection." in: Journal of virology, Vol. 81, Issue 10, pp. 5036-45, (2007) (PubMed).

Target Details for LDLR

Target: LDLR (Low Density Lipoprotein Receptor (LDLR))

Alternative Name: LDLR

Background: The low density lipoprotein receptor (LDLR) gene family consists of cell surface proteins involved in receptor-mediated endocytosis of specific ligands. Low density lipoprotein (LDL) is normally bound at the cell membrane and taken into the cell ending up in lysosomes where the protein is degraded and the cholesterol is made available for repression of microsomal enzyme 3-hydroxy-3-methylglutaryl coenzyme A (HMG CoA) reductase, the rate-limiting step in cholesterol synthesis. At the same time, a reciprocal stimulation of cholesterol ester synthesis takes place. Mutations in this gene cause the autosomal dominant disorder, familial hypercholesterolemiaSynonyms: LDL receptor, LDLR, Low-density lipoprotein receptor

Gene ID: 3949

NCBI Accession: NP_000518

UniProt: P01130

Pathways: Hepatitis C, Lipid Metabolism