Keratin 33A (KRT33A) antibody

Details for Product No. ABIN113469
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Antigen
Synonyms Ka27, RGD1306249, KRT33A, HA3I, Ha-3I, K33A, KRTHA3A, Krt1-3, hHa3-I, 2310015J09Rik, krtha3a
Reactivity
Human, Mouse (Murine)
(9), (1), (1)
Host
Guinea Pig
(8), (2)
Clonality
Polyclonal
Conjugate
Un-conjugated
(1), (1), (1), (1), (1), (1)
Application
Immunohistochemistry (Frozen Sections) (IHC (fro)), Western Blotting (WB)
(10), (6), (1), (1), (1)
Pubmed 2 references available
Quantity 0.1 mL
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Catalog No. ABIN113469
500.50 $
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Immunogen Synthetic peptide of human hair (trichocytic) keratin hHa3 (EKP IGS CVT NPC GPR SR), coupled to KLH.
Specificity The antiserum stains specifically Human hair keratin K33 expressed starting from the middle hair cortex. Because of high sequence homology both Keratin isoforms K33a (formerly also designated keratin hHa3-I) and K33b (formerly designated keratin hHa3-II) are detected.
Purification Liquid serum
Alternative Name Keratin-33A (KRT33A)
Background Synonyms: HHA3-I, HKA3A, Hair keratin type I Ha3-I, K33A, KRTHA3A, Keratin type I cuticular Ha3-I
Gene ID 3883
NCBI Accession NP_004129
UniProt O76009
Application Notes Immunohistochemistry on frozen sections: 1/200, 1h at RT, preincubate fixed sections with0.1 Triton X-100 (in PBS) for 1 - 5 min prior to first antibody incubation step. Immunoblotting: 1 : 2,000, ECL method.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Format Liquid
Storage 4 °C
Storage Comment Store the antibody undiluted at 2-8 °C.
Background publications Langbein, Rogers, Winter et al.: "The catalog of human hair keratins. I. Expression of the nine type I members in the hair follicle." in: The Journal of biological chemistry, Vol. 274, Issue 28, pp. 19874-84, 1999 (PubMed).

Langbein, Spring, Rogers et al.: "Hair keratins and hair follicle-specific epithelial keratins." in: Methods in cell biology, Vol. 78, pp. 413-51, 2005 (PubMed).

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