ENOS antibody

Catalog No. ABIN113627

The Rabbit Polyclonal anti-ENOS antibody is suitable to detect ENOS in samples from Human and Rat. It has been validated for WB, IF, IHC (fro) and EIA.

Quick Overview for ENOS antibody (ABIN113627)

Target: ENOS (NOS3) (Nitric Oxide Synthase 3 (Endothelial Cell) (NOS3))

Reactivity: Human, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ENOS antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Frozen Sections) (IHC (fro)), Enzyme Immunoassay (EIA)

Product Details anti-ENOS Antibody

Specificity: Specific for the amino terminal of eNOS. It recognizes the non-myrisylated form best, but does cross-react ~50 % with the myristylated form of eNOS. Also recognizes Rat eNOS. Cross-reactivity: eNOS (2-11 Cys12): 100 % Recombinant eNOS (NOS type III): 100 % eNOS (cap-2-11Cys12): 50 % Recombinant myristylated eNOS (myristylated NOS type III): 0 % Recombinant hnNOS (NOS type I): 0 % Recombinant hiNOS (NOS type II): 0 %

Cross-Reactivity (Details): Species reactivity (tested):Human and Rat.

Immunogen: eNOS (2-11 Cys12) covalently attached to a carrier protein

Application Details

Application Notes: Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Format: Liquid

Reconstitution: Restore with 0.1 mL of PBS which contains 10 mg/mL BSA or with additional buffer for more dilute antibody.

Preservative: Azide free

Storage: 4 °C

Storage Comment: Store the antibody undiluted at 2-8 °C. DO NOT FREEZE!. Avoid prolonged exposure to light.

Target Details for ENOS

Target: ENOS (NOS3) (Nitric Oxide Synthase 3 (Endothelial Cell) (NOS3))

Alternative Name: NOS3

Background: Nitric oxide synthase NOS oxidizes a guanidine nitrogen of arginine releasing nitric oxide in the form of a free radical and citrulline. Nitric oxide thus generated acts as a messenger in diverse functions including vasodilation neurotransmission, anti tumor and anti pathogenic activities. NOS is classified under three types: neuronal NOS (nNOS) or brain NOS (bNOS), inducible NOS (iNOS) or macrophage NOS (mNOS), and endothelial NOS (eNOS). eNOS is a calcium/calmodulin dependent enzyme which undergoes several post translational modifications, including acylation with myristate and palmitate, and phosphorylation on numerous residues. As with the other members of the NOS family, eNOS derives the diffusible multifunctional second messenger NO from L arginine through a series of reactions in which L citrulline is an intermediate. eNOS plays an important role in controlling vascular tone, platelet aggregation, and cardiac myocyte function.Synonyms: Constitutive NOS, EC-NOS, Endothelial NOS, NOS type III, Nitric oxide synthase, eNOS, endothelial

Gene ID: 4846

NCBI Accession: NP_000594

UniProt: P29474

Pathways: ACE Inhibitor Pathway, Regulation of Systemic Arterial Blood Pressure by Hormones, Cellular Response to Molecule of Bacterial Origin, Myometrial Relaxation and Contraction, Signaling Events mediated by VEGFR1 and VEGFR2, Thromboxane A2 Receptor Signaling, VEGFR1 Specific Signals, VEGF Signaling