CD72 Molecule (CD72) antibody (FITC)

Details for Product No. ABIN114239
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Antigen
Synonyms LYB2, CD72b
Reactivity
Mouse (Murine)
(64), (30)
Host
Mouse
(79), (17), (1)
Clonality (Clone)
Monoclonal ()
Conjugate
FITC
(16), (13), (12)
Application
Flow Cytometry (FACS)
(66), (20), (20), (13), (10), (4), (3), (1), (1), (1)
Pubmed 4 references available
Catalog no. ABIN114239
Quantity 0.3 mg
Price
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Clone CT-72-1
Isotype IgG2a
Specificity This antibody reacts with the CD72 alloantigen CD72.1, a B-cell surface protein that is encoded by the Cd72a allele. CD72.1 is expressed on cells of the B cell lineage, except plasma cells3. Mouse strains expressing CD72.1 include C57L/-, C58/-, DBA/1, DBA/2, and SWR/J. Tissue Distribution by Flow Cytometry Analysis: (Representative Dot Plot) Mouse Strain Tested: DBA/2 Cell Concentration : 1x10e6 cells per test Antibody Concentration Used: 0.25 µg/10e6 cells Isotypic Control: FITC Mouse IgG2a.
Alternative Name CD72
Background CD72 antigen is a member of the type II integral membrane glycoproteins which includes other related cell surface molecules such as the asialoglycoprotein receptors, CD23 and the Kupffer cell receptor. The function of CD72 is unknown but the exposure of B cells to CD72 antibodies activates a variety of signaling pathways and can induce MHC class II expression and B cell proliferation. CD72 antigen is expressed on all cells of B cell lineage with the exception of plasma cells and weakly on human tissue macrophages.
Alternate names: B-Cell marker, B-cell differentiation antigen CD72, Ly-32, Ly32, Lyb-2, Lymphocyte antigen 32
Gene ID 100504743
NCBI Accession XP_003086403.1
UniProt P21855
Research Area Stem Cells, Hematopoietic Progenitors, Adaptive Immunity, CD Antigens, Surface Receptors of Immune Cells
Application Notes Flow cytometry (For details please see protocols / specifity below). Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Protocol FLOW CYTOMETRY ANALYSIS: Method: 1. Prepare a cell suspension in media A. For cell preparations, deplete the red blood cellpopulation. 2. Wash 2 times. 3. Resuspend the cells to a concentration of 2x10e7 cells/ml in media A. Add 50 µl of thissuspension to each tube (each tube will then contain 1 x 10e6 cells, representing 1 test). 4. To each tube, add ~0. 25 µg of antibody per 1 x 10e6 cells. 5. Vortex the tubes to ensure thorough mixing of antibody and cells. 6. Incubate the tubes for 30 minutes at 4°C. (It is recommended that the tubes areprotected from light, since most fluorochromes are light sensitive. )7. Wash 2 times at 4°C. 8. Resuspend the cell pellet in 50 µl ice cold media B. 9. Transfer to suitable tubes for flow cytometric analysis containing 15 µl of propidiumiodide at 0. 5 mg/ml in PBS. This stains dead cells by intercalating in DNA. Media: A. Phosphate buffered saline (pH 7. 2) + 5% normal serum of host species + sodium azide(100 µl of 2M sodium azide in 100 mls). B. Phosphate buffered saline (pH 7. 2) + 0. 5% Bovine serum albumin + sodium azide (100µl of 2M sodium azide in 100 mls).
Restrictions For Research Use only
Format Liquid
Concentration 0.1 mg/mL
Buffer PBS, 0.09% Sodium azide and EIA grade BSA as a stabilizing protein to bring total protein concentration to 4-5 mg/ml.
Preservative Sodium azide
Handling Advice Dilute only prior to immediate use. Avoid cycles of freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store the antibody (undiluted) at 2-8°C for one month or (in aliquots) at -20°C for longer.
Expiry Date 12 months
Supplier Images
anti-CD72 Molecule (CD72) antibody (FITC) Lymphozytes FL1-H: Mouse anti mouse CD72.1, FL2-H: B222 PE
Background publications Luo, Van de Velde, von Hoegen et al.: "Ly-1 (CD5), a membrane glycoprotein of mouse T lymphocytes and a subset of B cells, is a natural ligand of the B cell surface protein Lyb-2 (CD72)." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 148, Issue 6, pp. 1630-4, 1992 (PubMed).

Robinson, Landolfi, Schäfer et al.: "Biochemical identity of the mouse Ly-19.2 and Ly-32.2 alloantigens with the B cell differentiation antigen Lyb-2/CD72." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 151, Issue 9, pp. 4764-72, 1993 (PubMed).

Ying, Healy, Goodnow et al.: "Regulation of mouse CD72 gene expression during B lymphocyte development." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 161, Issue 9, pp. 4760-7, 1998 (PubMed).

Schimmel, Kearley, Nijkamp et al.: "Hydrogen adsorption in carbon nanostructures: comparison of nanotubes, fibers, and coals." in: Chemistry (Weinheim an der Bergstrasse, Germany), Vol. 9, Issue 19, pp. 4764-70, 2003 (PubMed).

Hosts (79), (17), (1)
Reactivities (64), (30)
Applications (66), (20), (20), (13), (10), (4), (3), (1), (1), (1)
Conjugates (16), (13), (12)
Epitopes (4), (2), (2), (2), (1), (1)
Request Want additional data for this product?

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Order hotline:

  • +1 404 474 4654
  • +1 888 205 9894 (TF)
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