CD72 antibody (CD72 Molecule)

Details for Product anti-CD72 Antibody No. ABIN94227
Antigen
Reactivity
Human
103
46
10
Host
Mouse
69
63
1
Clonality (Clone)
Monoclonal ()
Conjugate
This CD72 antibody is un-conjugated
17
12
11
4
3
3
1
1
1
1
1
1
Application
Flow Cytometry (FACS), Immunoprecipitation (IP)
68
54
44
18
13
7
6
4
2
2
2
1
1
1
Options
Immunogen Normal human lymphocytes from a lymph node.
Clone 3F3
Isotype IgG2b
Specificity The antibody 3F3 reacts with CD72, a 39-43 kDa type II membrane glycoprotein (C-type lectin family). CD72 is a pan-B cell marker expressed throughout the B lymphocytes diferentiation with the exception of plasma cells, it is also present on follicular dendritic cells.
HLDA V, WS Code B CD72.5
HLDA VI, WS Code B CD72.1
HLDA VI, WS Code 6 BP 84
Cross-Reactivity (Details) Human
Purification Purified by precipitation and chromatography
Purity > 95 % (by SDS-PAGE)
Antigen
Alternative Name CD72 (CD72 Antibody Abstract)
Background CD72 is a transmembrane glycoprotein expressed as a homodimer especially in B cells, but also in other antigen presenting cells such as dendritic cells and macrophages. Through one of its immunoreceptor tyrosine-based inhibitory motives (ITIMs), CD72 interacts with tyrosine phosphatase SHP-1, thereby suppressing B cell responsiveness. Binding of CD72 with its ligand CD100 (Sema4D) prevents BCR association and phosphorylation of CD72 and results in dissociation of SHP-1 from CD72, thus enables B cell activation.
Application Notes Optimal working dilution should be determined by the investigator.
Restrictions For Research Use only
Concentration 1 mg/mL
Buffer Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Do not freeze.
Storage 4 °C
Storage Comment Store at 2-8°C. Do not freeze. Do not use after expiration date stamped on vial label.
Supplier Images
Image no. 1 for anti-CD72 Molecule (CD72) antibody (ABIN94227) Surface staining of CD72 on human peripheral blood cells with anti-CD72 (3F3) purifie...
Product cited in: Mizrahi, Markel, Porgador, Bushkin, Mandelboim: "CD100 on NK cells enhance IFNgamma secretion and killing of target cells expressing CD72." in: PLoS ONE, Vol. 2, Issue 9, pp. e818, 2007 (PubMed).