GFAP antibody

Catalog No. ABIN114688

This Mouse Monoclonal antibody specifically detects GFAP in WB, IHC (p) and IHC (fro). It exhibits reactivity toward Human and Mouse.

Quick Overview for GFAP antibody (ABIN114688)

Target: GFAP (Glial Fibrillary Acidic Protein (GFAP))

Reactivity: Human, Mouse

Host: Mouse

Clonality: Monoclonal

Conjugate: This GFAP antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Clone: 6F2

Product Details anti-GFAP Antibody

Specificity: This antibody 6F2 reacts exclusively with glial fibrillary acidic protein which is present in astrocytes in the central nervous system and Schwann cells.

Cross-Reactivity (Details): Species reactivity (tested):Human, Mouse.

Purification: Purified

Immunogen: Glial fibrillary acidic protein from human brain.

Isotype: IgG1

Application Details

Application Notes: Suitable for Immunoblotting and Immunohistochemistry on frozen sections andparaffin-embedded tissues. Recommended dilutions: 1/25-1/200 for Immunohistochemistry with avidin-biotinylatedhorseradish peroxidase complex (ABC) as detection reagent, and 1/100-1/1000 forImmunoblotting applications.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: PBS, 0.09 % Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store lyophilized at 2-8 °C and reconstituted at -20 °C.

Target Details for GFAP

Target: GFAP (Glial Fibrillary Acidic Protein (GFAP))

Alternative Name: GFAP

Background: GFAP (55 kD) is selectively located in astrocytes and represents the major constituent of astrocytic intermediate filaments. GFAP expression levels are highly variable during development of the central nervous system. In adults, GFAP levels increase as a result of the proliferation of astrocytes that occurs in a response to a variety of physical, chemical and etiological insults, including Alzheimer's disease, epilepsy and multiple sclerosis. In the peripheral nervous system GFAP is expressed by Schwann cells. Upon differentiation, myelin forming Schwann cells down-regulate GFAP, whereas in non-myelin forming Schwann cells GFAP persists into adulthood.Synonyms: Glial Fibrillary Acidic Protein

Gene ID: 2670

NCBI Accession: NP_001229305

UniProt: P14136