CCL2 antibody (Biotin)

Catalog No. ABIN114714

The Biotin-conjugated Mouse Monoclonal anti-CCL2 antibody (Clone S101) (ABIN114714) specifically detects CCL2 in EIA. The antibody is reactive with Human samples.

Quick Overview for CCL2 antibody (Biotin) (ABIN114714)

Target: CCL2 (Chemokine (C-C Motif) Ligand 2 (CCL2))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CCL2 antibody is conjugated to Biotin

Application: Enzyme Immunoassay (EIA)

Clone: S101

Product Details anti-CCL2 Antibody (Biotin)

Purification: Protein G affinity purified

Immunogen: Purified recombinant human MCP-1

Isotype: IgG1

Application Details

Application Notes: ELISA: This Biotin-conjugated monoclonal antibody can be used as a tracer/detectionantibody in sandwich ELISA applications for human MCP-1 detection in combination with acapture antibody. Suggested Capture Coating Dose: approx. 0.3g/mL, Substrate: TMB. If theabove suggested conditions are followed approximately 1.5 pg/mL of MCP-1 inserum/plasma or 4 pg/mL of MCP-1 in medium can be detected with an assay range of0-1600 pg/mL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: 0.01 M PBS, pH 7.0±0.1, 0.1 % Proclin-300, 1 % Gelatin

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: -20 °C

Target Details for CCL2

Target: CCL2 (Chemokine (C-C Motif) Ligand 2 (CCL2))

Alternative Name: MCP1 / CCL2

Background: Monocyte chemotactic and activating factor (MCAF) is also called monocyte chemotactic protein-1 (MCP-1) and chemokine (C-C motif) ligand 2 (CCL2). It is primarily secreted by monocytes, macrophages and dendritic cells. This cytokine displays chemotactic activity for monocytes, T-cells, and basophils, but not for neutrophils or eosinophils. MCAF causes the degranulation of basophils and mast cells, and augments the activity of monocyte and macrophage. MCAF plays an important role in inflammation, angiogenesis, auto-immune diseases, renal diseases, chronic infection and granuloma formation.Synonyms: C-C motif chemokine 2, HC-11, HC11, MCAF, MCP-1, Monocyte chemoattractant protein 1, Monocyte chemotactic and activating factor, Monocyte chemotactic protein 1, Monocyte secretory protein JE, SCYA2, Small-inducible cytokine A2

Gene ID: 9606

UniProt: P13500

Pathways: Cellular Response to Molecule of Bacterial Origin, Positive Regulation of Immune Effector Process, ER-Nucleus Signaling, Unfolded Protein Response, The Global Phosphorylation Landscape of SARS-CoV-2 Infection