Reactive with natural and recombinant Human MCP-3. Does not show any cross reaction with other Human Cytokines or growth factors tested such as IL1 beta, IL-8, MCAF, SAA and EGF.
CCL7
Reactivity: Rat
IHC
Host: Rabbit
Polyclonal
unconjugated
Application Notes
ELISA. Western Blot: CCL7 antibody concentration of 1-4 μg/mL will allow visualization of 0.1 μg/lane of human MCP-3. Matched Pair: DM2035 Clone 113DM2039 Clone 542 Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions
For Research Use only
Reconstitution
Restore with Double distillated water to adjust the final concentration to 1.0 mg/mL
Buffer
0.01 M PBS, pH 7.2 without preservatives
Preservative
Without preservative
Handling Advice
This product is photosensitive and should be protected from light
Storage
4 °C
Storage Comment
Store the antibody at 2 - 8 °C. DO NOT FREEZE! If the reagent is being diluted, it is recommended that only the quantity to be used withing one week be diluted.
Macrophage/monocyte chemotactic protein-3 (MCP-3), like other two closely related chemokines (MCP1 and MCP-2), is chemotactic to monocytes, but not to neutrophils. MCP-3 showed 78 % sequence homology with MCP-1 and 58 % sequence homology with MCP-2. The primary receptor for MCP-3 is D6. MCP-3 also competes with MCP-1 to bind to CCR10, and competes with Rantes and MIP-1α to bind to CCR1. MCP-3 stimulates macrophage to secrete proteases. A variety of cancer cell lines express MCP-3, suggesting a role in tumor invasion and metastasia.Synonyms: C-C motif chemokine 7, CCL-7, MCP-3, Macrophage Chemotactic Protein-3, Monocyte chemoattractant protein 3, Monocyte chemotactic protein 3, NC28, SCYA6, SCYA7, Small-inducible cytokine A7