CCL3 antibody

Catalog No. ABIN114724

This Mouse Monoclonal antibody specifically detects CCL3 in EIA. It exhibits reactivity toward Human.

Quick Overview for CCL3 antibody (ABIN114724)

Target: CCL3 (Chemokine (C-C Motif) Ligand 3 (CCL3))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CCL3 antibody is un-conjugated

Application: Enzyme Immunoassay (EIA)

Clone: 116

Product Details anti-CCL3 Antibody

Specificity: This antibody reacts with recombinant MIP-1α. Does not show any cross reaction with other human cytokines or growth factors tested such as M-CSF, GM-CSF, IL-7, IL-8, IL-16, IL-1β, bFGF, TGF-β, EGF, TNF-α and EPO.

Purification: Protein G affinity chromatography

Immunogen: Purified recombinant human MIP-1alpha

Isotype: IgG2a

Application Details

Application Notes: ELISA. Successful use in Western blot was reported by some researchers. As this detectionmethod has not been verified by antibodies-online, the application was deleted from thedatabase. This does not necessarily exclude the use in such procedure.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Reconstitution: Restore with double distillated water to adjust the final concentration to 1.0 mg/mL.

Buffer: 0.01 M PBS, pH 7.2

Handling Advice: Avoid repeated freezing and thawing.

Storage: -20 °C

Storage Comment: Store the antibody at -20 °C. Can be shipped at 2 - 8 °C.

Target Details for CCL3

Target: CCL3 (Chemokine (C-C Motif) Ligand 3 (CCL3))

Alternative Name: MIP1 alpha / CCL3

Background: Macrophage Inflammatory Protein 1 alpha (MIP1 alpha) and MIP1 beta, two closely related but distinct proteins, were originally co-purified from medium conditioned by a LPS-stimulated murine macrophage cell line. Mature mouse MIP1 alpha shares approximately 77 % and 70 % amino acid identity with human MIP1 alpha and mouse MIP1 beta, respectively. MIP1 proteins are expressed primarily in T cells, B cells, and monocytes after antigen or mitogen stimulation. Has adhesive effects on lymphocytes. MIP1 alpha can inhibit the proliferation of hematopoietic stem cells in vitro as well as in vivo. A signal transducing receptor, designated the C-C chemokine receptor-1 (C-C CKR-1) with seven transmembrane domains that bind MIP1 alpha, MIP1 beta, MCP1 and RANTES with varying affinities, has been isolated.Synonyms: C-C motif chemokine 3, CCL-3, G0/G1 switch regulatory protein 19-1, G0S19-1 protein, LD78 alpha, MIP-1-alpha, MIP1A, Macrophage Inflammatory Protein 1 alpha, SCYA3, Small-inducible cytokine A3, Tonsillar lymphocyte LD78 alpha protein

Gene ID: 6348

NCBI Accession: NP_002974

UniProt: P10147

Pathways: Cellular Response to Molecule of Bacterial Origin, Autophagy