MYOD1 antibody

Catalog No. ABIN114805

This Mouse Monoclonal antibody specifically detects MYOD1 in IP, IHC (p) and IHC (fro). It exhibits reactivity toward Human, Mouse, Rat and Chicken.

Quick Overview for MYOD1 antibody (ABIN114805)

Target: MYOD1 (Myogenic Differentiation 1 (MYOD1))

Reactivity: Human, Mouse, Rat, Chicken

Host: Mouse

Clonality: Monoclonal

Conjugate: This MYOD1 antibody is un-conjugated

Application: Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Clone: 5-2F

Product Details anti-MYOD1 Antibody

Purification: Purified

Immunogen: BALB/C mice were injected with a recombinant mouse MyoD1 protein.

Isotype: IgG2a

Application Details

Application Notes: Immunoprecipitation: 1/50Immunohistochemistry on Formalin-Fixed Paraffin Embedded Sections: 1/25-1/50 in an ABCmethod for 30 minutes at room temperature. Formalin fixed paraffin embedded tissue sections require high temperature antigenunmasking with 1 mM EDTA, pH 8.0 or 10 mM Citrate buffer, pH 6.0 prior to immunostaining. Recommended Positive Control: Rhabdomyosarcoma
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Format: Liquid

Storage: 4 °C

Storage Comment: Store the antibody undiluted at 2-8 °C.

Target Details for MYOD1

Target: MYOD1 (Myogenic Differentiation 1 (MYOD1))

Alternative Name: MYOD1

Background: MyoD1 belongs to the basic helix-loop-helix family of transcription factors and the myogenic factors subfamily. It regulates muscle cell differentiation by inducing cell cycle arrest, a prerequisite for myogenic initiation. Myod1 is essential for repair of damaged tissue. It activates its own transcription which may stabilize commitment to myogenesis.Synonyms: BHLHC1, Class C basic helix-loop-helix protein 1, MYF3, MYOD, Myoblast determination protein 1, Myogenic factor 3

Gene ID: 10090

UniProt: P10085

Pathways: Regulation of Muscle Cell Differentiation, Skeletal Muscle Fiber Development