Add to Basket
Order hotline:
 |
+1 404 474 4654 |
 |
+1 888 205 9894 (TF) |
Ezrin (EZR) antibody
| Antigen | Ezrin (EZR) |
| Synonyms | CVL, CVIL, VIL2, MGC1584, FLJ26216, DKFZp762H157, EZR, vil2, wu:fc05c03, wu:fj31d06, cvl, cvil, MGC97499 |
| Clonality | Monoclonal (3C12) |
| Host |
 Alternatives Mouse |
| Reactivity |
Alternatives Cow (Bovine), Hamster, Human, Mouse (Murine), Rat (Rattus) |
| Application |
Alternatives Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
|
 |
3 references available |
| Catalog no. | ABIN115202 |
| Quantity | 0,5 ml (Variants) |
| Price | 330.00 $ Plus shipping costs $35.00 |
| Shipping to |
|
| Availability | Ships within 7 to 10 Business Days |
Additional Information
| Characteristics | Synonyms: EZR, VIL2, p81, Cytovillin, Villin-2, Villin2, VIL-2 |
| Alternative name | Ezrin |
| Swiss-Prot | P15311 |
| Immunogen | BALB/C mice were immunized with a recombinant fragment of human ezrin correspondingto amino acid 362-585. |
| Isotype | IgG1 (Matching secondary antibodies) |
| Clone | 3C12 |
| Description | Ezrin serves as a tyrosine kinase substrate and is phosphorylated in EGF-stimulated cells. Ezrin is a cytoplasmic protein enriched in microvilli and other cell surfaces. Ezrin has anactin-binding capacity. Ezrin is expressed in epithelial cells but not in mesenchymal cells. Ezrin is also expressed by certain epithelial tumors, such as renal cell adenocarcinomas. |
| Specificity | This antibody reacts with a protein of 81 kD. Cellular Localization: Cell membraneSpecies: Human, Monkey, Cow, Kangaroo, Rat, Mouse, Hamster. Others not tested. |
Application Details
| Application Notes | Immunoprecipitation. Western blot: 1: 100-750. Immunohistochemistry on paraffin embedded sections: dilute 1: 50-1: 100 in an ABC method(30 minutes at room temperature). Recommended positive control: Lung. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user. |
| Storage | Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing. Shelf life: one year from despatch. |
| Research Area | Cancer, Cytoskeleton, Microfilaments |
| Restrictions | For Research Use only |
Publications
| Publications |
Turunen, Wahlstroem, Vaheri: "Ezrin has a COOH-terminal actin-binding site that is conserved in the ezrin protein family." in: The Journal of cell biology, Vol. 126, Issue 6, pp. 1445-53, 1994 (PubMed).
Boehling, Turunen, Jaeaeskelaeinen et al.: "Ezrin expression in stromal cells of capillary hemangioblastoma. An immunohistochemical survey of brain tumors." in: The American journal of pathology, Vol. 148, Issue 2, pp. 367-73, 1996 (PubMed). Helander, Carpuen, Turunen et al.: "ICAM-2 redistributed by ezrin as a target for killer cells." in: Nature, Vol. 382, Issue 6588, pp. 265-8, 1996 (PubMed). |
Alternatives
Alternatives for antigen "Ezrin (EZR)", type "Antibodies"
| Hosts | Rabbit (67), Goat (6), Mouse (3) |
| Reactivities | Human (75), Mouse (Murine) (61), Rat (Rattus) (53), Dog (Canine) (4), Cow (Bovine) (2), Chicken (1), Hamster (1), Rabbit (1) |
| Applications | Western Blotting (WB) (56), Flow Cytometry (FACS) (20), Immunofluorescence (IF) (18), ELISA (11), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (10), Immunohistochemistry (IHC) (8), Enzyme Immunoassay (EIA) (6), Immunoprecipitation (IP) (4), Immunocytochemistry (ICC) (2), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (2), Immunoelectron Microscopy (IEM) (1) |
| Conjugates | Alexa Flour 488 (1), Alexa Fluor 350 (1), Alexa Fluor 488 (1), Alexa Fluor 555 (1), Alexa Fluor 647 (1), Biotin (1), Cy3 (1), Cy5 (1), Cy5.5 (1), Cy7 (1), FITC (1), Gold (1), HRP (1), PE (1), PE,Cy3 (1), PE,Cy5 (1), PE,Cy5.5 (1), PE,Cy7 (1), RBITC (1) |
| Epitopes | pThr566 (6), pTyr353 (4), pThr567 (2), pSer566 (1), pTyr146 (1), pTyr478 (1) |
