Ezrin antibody (N-Term)

Catalog No. ABIN7603214

The Rabbit Polyclonal anti-Ezrin antibody (ABIN7603214) specifically detects Ezrin in WB, IHC, IF, FACS and ICC. The antibody is reactive with Human, Mouse, Rat and Monkey samples.

Quick Overview for Ezrin antibody (N-Term) (ABIN7603214)

Target: Ezrin (EZR)

Reactivity: Human, Mouse, Rat, Monkey

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Ezrin antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS), Immunocytochemistry (ICC)

Product Details anti-Ezrin Antibody

Binding Specificity: N-Term

Purpose: Anti-Ezrin/EZR Antibody

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-Ezrin/EZR Antibody. Tested in Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat, Monkey.

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the N-terminus of human Ezrin/EZR, which shares 85.7% amino acid (aa) sequence identity with mouse and rat EZR.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.1-0.25 μg/mL, Human, Mouse, Rat, Monkey Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/mL, Human, Rat Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for Ezrin

Target: Ezrin (EZR)

Alternative Name: EZR

Background:

Background: Ezrin also known as cytovillin or villin-2 is a protein that in humans is encoded by the EZR gene. The cytoplasmic peripheral membrane protein encoded by this gene functions as a protein-tyrosine kinase substrate in microvilli. As a member of the ERM protein family, this protein serves as an intermediate between the plasma membrane and the actin cytoskeleton. This protein plays a key role in cell surface structure adhesion, migration and organization, and it has been implicated in various human cancers. A pseudogene located on chromosome 3 has been identified for this gene. Alternatively spliced variants have also been described for this gene.

Gene Full Name: ezrin

Molecular Weight: 81 kDa

Gene ID: 7430

UniProt: P15311

Pathways: Maintenance of Protein Location