Cytokeratin 1 antibody (pan)

Catalog No. ABIN115296

The Rabbit Polyclonal anti-Cytokeratin 1 antibody (ABIN115296) specifically detects Cytokeratin 1 in IHC (p). The antibody is reactive with Human samples.

Quick Overview for Cytokeratin 1 antibody (pan) (ABIN115296)

Target: Cytokeratin 1 (KRT1) (Keratin 1 (KRT1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Cytokeratin 1 antibody is un-conjugated

Application: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-Cytokeratin 1 Antibody

Binding Specificity: pan

Specificity: This antibody reacts with Cytokeratins of 68, 60, 58, 56, 52, 51, and 68 kDa MW. It is well suited for the staining of a broad spectrum of keratins. Cellular localization: cytoplasmic.

Cross-Reactivity (Details): Species reactivity (tested):Human.

Purification: Purified

Immunogen: Cytokeratin isolated from bovine muzzle epidermis

Application Details

Application Notes: Immunohistochemistry on Formalin-Fixed Paraffin Embedded Tissues: This antibody maybe diluted to a titer of 1/75-1/200 in an ABC method (30 minutes at room temperature). Proteolytic enzymatic pretreatment of formalin fixed paraffin embedded sections isrecommended prior to immunostaining. Recommended Positive Control: skin.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Format: Liquid

Storage: 4 °C

Storage Comment: Store the antibody undiluted at 2-8 °C.

Target Details for Cytokeratin 1

Target: Cytokeratin 1 (KRT1) (Keratin 1 (KRT1))

Alternative Name: Cytokeratin

Background: Cytokeratins, a group comprising at least 29 different proteins, are characteristic of epithelial and trichocytic cells. Cytokeratins 1, 4, 5, 6, and 8 are members of the type II neutral to basic subfamily. Monoclonal anti cytokeratins are specific markers of epithelial cell differentiation and have been widely used as tools in tumor identification and classification. Monoclonal Anti Pan Cytokeratin (mixture) is a broadly reactive reagent, which recognizes epitopes present in most human epithelial tissues. It facilitates typing of normal, metaplastic and neoplastic cells. Synergy between the various components results in staining amplification. This enables identification of cells, which would otherwise be stained only marginally. The mixture may aid in the discrimination of carcinomas and nonepithelial tumors such as sarcomas, lymphomas and neural tumors. It is also useful in detecting micrometastases in lymph nodes, bone marrow and other tissues and for determining the origin of poorly differentiated tumors. There are two types of cytokeratins the acidic type I cytokeratins and the basic or neutral type II cytokeratins. Cytokeratins are usually found in pairs comprising a type I cytokeratin and a type II cytokeratin. Usually the type II cytokeratins are 8kD larger than their type I counterparts.Synonyms: Cytokeratin pan-reactive, pan Keratin