Rabbit anti-Human IgE Antibody

Catalog No. ABIN115320

Quick Overview for Rabbit anti-Human IgE Antibody (ABIN115320)

Target: IgE

Clonality: Polyclonal

Application: Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details

Reactivity: Human

Host: Rabbit

Purification: Purified

Immunogen: IgE isolated from the serum of a patient with IgE-myeloma.

Application Details

Application Notes: Immunohistochemistry on Formalin-Fixed Praffin Embedded Sections: Use at a dilution of1/50-1/500 in an ABC method (30 minutes at room temperature) Recommended Positive Control: Tonsil.

Other applications not tested.
Otimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Format: Liquid

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -28 °C for longer.

Target Details

Target: IgE

Background: IgE is typically the least abundant isotype - blood serum IgE levels in a normal ("non-atopic") individual are ~150 ng/mL, compared to 10 mg/mL for the IgGs - it is capable of triggering the most powerful immune reactions. Most of our knowledge of IgE has come from research into the mechanism of a form of allergy known as type 1 hypersensitivity. There is much speculation into what physiological benefits IgE contributes, and so far, circumstantial evidence in animal models and statistical population trends have hinted that IgE may be beneficial in fighting gut parasites such as Schistosoma mansoni, but this has not been conclusively proven in humans. IgE may play an important role in the immune system's recognition of cancer, in which the stimulation of a strong cytotoxic response against cells displaying only small amounts of early cancer markers would be beneficial. IgE may be an important target in treatments for allergy and asthma.Synonyms: Immunoglobulin E human