CCL22 antibody

Catalog No. ABIN115780

This Mouse Monoclonal antibody specifically detects CCL22 in WB, EIA and Func. It exhibits reactivity toward Human.

Quick Overview for CCL22 antibody (ABIN115780)

Target: CCL22 (Chemokine (C-C Motif) Ligand 22 (CCL22))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CCL22 antibody is un-conjugated

Application: Western Blotting (WB), Enzyme Immunoassay (EIA), Functional Studies (Func)

Product Details anti-CCL22 Antibody

Specificity: Recognises Human Macrophage Derived Chemokine (MDC)

Purification: Affinity Chromatography on Protein A

Immunogen: highly pure (>98%) recombinant Human MDC

Isotype: IgG1

Application Details

Application Notes: ELISA: In a sandwich ELISA (assuming 100 μL/well), a concentration of 2-4 μg/mL of thisantibody will detect at least 100 pg/mL of recombinant Human MDC when used withbiotinylated antigen affinity purified anti-Human MDC antibody (cat. PP1049B) as thedetection antibody at a concentration of at least 1 μg/mL. Western Blot: To detect Human MDC by Western Blot analysis this antibody can be used ata concentration of 0.25-0.50 μg/mL. Used in conjunction with compatible secondaryreagents the detection limit for recombinant hMDC is 0.25-0.50 ng/lane, undernon-reducing conditions. Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity ofhMIG (100 ng/mL), a concentration of 5.0-10.0 μg/mL of this antibody is required.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Reconstitution: Restore in sterile water to a concentration of 1.0 mg/mL.

Buffer: PBS without preservatives

Preservative: Without preservative

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody prior to reconstitution at -20 °C. Following reconstitution the antibody can be stored at 2-8 °C for one month or at -20 °C for longer.

Target Details for CCL22

Target: CCL22 (Chemokine (C-C Motif) Ligand 22 (CCL22))

Alternative Name: MDC / CCL22

Background: The MDC gene is one of several Cys-Cys (CC) cytokine genes which are clustered on the q arm of chromosome 16. Cytokines are a family of secreted proteins involved in immunoregulatory and inflammatory processes. The CC cytokines are proteins characterized by two adjacent cysteines. The cytokine encoded by MDC displays chemotactic activity for natural killer cells, chronically activated T lymphocytes, monocytes and dendritic cells. It has no chemoattractant activity for eosinophils, neutrophils and resting T lymphocytes and also displays a mild activity for primary activated T lymphocytes. The product of this gene binds to chemokine receptor CCR4. This chemokine may play a role in the trafficking of activated T lymphocytes to inflammatory sites and other aspects of activated T lymphocyte physiology.Synonyms: C-C motif chemokine 22, CC chemokine STCP-1, Macrophage-derived chemokine, SCYA22, Small-inducible cytokine A22, Stimulated T-cell chemotactic protein 1

Gene ID: 6367

NCBI Accession: NP_002981

UniProt: O00626