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Antibodies
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anti-Lymphotoxin alpha (TNF Superfamily, Member 1) (LTA) antibody (Biotin)
Lymphotoxin alpha (TNF Superfamily, Member 1) (LTA) antibody (Biotin)
| Antigen |
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| Synonyms |
LT, TNFB, TNFSF1, Ltx, Tnfb, LT[a], LT-[a], hlb382, LTalpha, Tnfsf1b, LT-alpha, TNF-beta, MGC117668, LT-a, tnfb, tnfsf1, LTA |
| Clonality |
Polyclonal |
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Host
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Reactivity
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Conjugate
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Biotin (2), FITC (2), Alexa Flour 488 (1), Alexa Fluor 350 (1), Alexa Fluor 488 (1), Alexa Fluor 555 (1), Alexa Fluor 647 (1), Cy3 (1), Cy5 (1), Cy5.5 (1), Cy7 (1), Gold (1), HRP (1), PE (1), PE,Cy3 (1), PE,Cy5 (1), PE,Cy5.5 (1), PE,Cy7 (1), RBITC (1)
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Application
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 Alternatives Enzyme Immunoassay (EIA), Western Blotting (WB)
Immunofluorescence (IF) (22), Flow Cytometry (FACS) (20), Western Blotting (WB) (19), Enzyme Immunoassay (EIA) (7), ELISA (6), Blocking Antibody (Inhibition) (3), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (3), Functional Studies (Func) (2), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (2), Immunoprecipitation (IP) (2), Immunoelectron Microscopy (IEM) (1), Immunohistochemistry (IHC) (1), Neutralization (Neut) (1)
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| Catalog no. |
ABIN116246 |
| Quantity |
50 µg (Variants) |
| Price |
400.00 $ Plus shipping costs $35.00
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| Shipping to |
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| Availability |
Ships within 7 to 10 Business Days |
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Characteristics
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Synonyms: TNFB, TNFSF1, LTA, LT-alpha, Tumor necrosis factor ligand superfamily member 1
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Alternative name
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TNF-beta / Lymphotoxin-alpha
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Swiss-Prot
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P01374
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Immunogen
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Highly pure (>98%) recombinant hTNF-beta.
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Specificity
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This antibody reacts with Tumor Necrosis Factor beta. Species: Human. Others not tested. Add. Information: Centrifuge vial prior to opening!
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Application Notes
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ELISA: (Direct): To detect hTNF-β by direct ELISA (using 100 μl/well antibody solution) aconcentration of 0.25-1.0 μg/ml of this antibody is required. This biotinylated polyclonalantibody, in conjunction with compatible secondary reagents, allows the detection of atleast 0.2-0.4 ng/well of recombinant hTNF-β. (Sandwich): To detect hTNF-β by sandwich ELISA (using 100 μl/well antibody solution) aconcentration of 0.25-1.0 μg/ml of this antibody is required. This biotinylated polyclonalantibody, in conjunction with Polyclonal Anti-Human TNF-β (PP1072P1/PP1072P2) as acapture antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant hTNF-β. Western Blot: To detect hTNF-β by Western Blot analysis this antibody can be used at aconcentration of 0.1-0.2 ug/ml. Used in conjunction with compatible secondary reagentsthe detection limit for recombinant hTNF-β is 1.5- 3.0 ng/lane, under either reducing ornon-reducing conditions. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
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Buffer
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PBS, pH 7.2 without preservatives.Label: Biotin conjugated.Reconstitution: Restore in sterile PBS containing 0.1% BSA to a concentration of 0.1-1.0mg/ml.
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Storage
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Store the antibody prior to reconstitution at -20°C. Following reconstitution the antibodycan be stored at 2-8°C for one month or at -20°C for longer. Avoid repeated freezing and thawing. Shelf life: One year from despatch.
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Research Area
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Cytokines, Growth Factors, Receptors, Immunology, Inflammation
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Restrictions
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For Research Use only
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Alternatives for antigen "Lymphotoxin alpha (TNF Superfamily, Member 1) (LTA)", type "Antibodies"
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Hosts
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Rabbit (33), Mouse (5)
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Reactivities
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Human (36), Mouse (Murine) (21), Rat (Rattus) (20), Cow (Bovine) (15), Dog (Canine) (15), Horse (Equine) (15), Rabbit (15)
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Applications
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Immunofluorescence (IF) (22), Flow Cytometry (FACS) (20), Western Blotting (WB) (19), Enzyme Immunoassay (EIA) (7), ELISA (6), Blocking Antibody (Inhibition) (3), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (3), Functional Studies (Func) (2), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (2), Immunoprecipitation (IP) (2), Immunoelectron Microscopy (IEM) (1), Immunohistochemistry (IHC) (1), Neutralization (Neut) (1)
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Conjugates
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Biotin (2), FITC (2), Alexa Flour 488 (1), Alexa Fluor 350 (1), Alexa Fluor 488 (1), Alexa Fluor 555 (1), Alexa Fluor 647 (1), Cy3 (1), Cy5 (1), Cy5.5 (1), Cy7 (1), Gold (1), HRP (1), PE (1), PE,Cy3 (1), PE,Cy5 (1), PE,Cy5.5 (1), PE,Cy7 (1), RBITC (1)
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Epitopes
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Center (1)
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