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Lymphotoxin alpha (TNF Superfamily, Member 1) (LTA) antibody

Antigen

Lymphotoxin alpha (TNF Superfamily, Member 1) (LTA)
Synonyms LT, TNFB, TNFSF1, Ltx, Tnfb, LT[a], LT-[a], hlb382, LTalpha, Tnfsf1b, LT-alpha, TNF-beta, MGC117668, LT-a, tnfb, tnfsf1, LTA
Clonality Polyclonal
Host
Alternatives

Rabbit
Reactivity
Alternatives

Human
Application
Alternatives Enzyme Immunoassay (EIA), Functional Studies (Func), Western Blotting (WB)
Catalog no. ABIN116248
Quantity 0.1 mg  (Variants)
Price 400.00 $   Plus shipping costs $35.00
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Availability Ships within 7 to 10 Business Days

Additional Information
Application Details Alternatives Secondary Antibodies Variants back to top

Characteristics Synonyms: TNFB, TNFSF1, LTA, LT-alpha, Tumor necrosis factor ligand superfamily member 1
Alternative name TNF-beta / Lymphotoxin-alpha
Swiss-Prot P01374
Immunogen Highly pure (>98%) recombinant hTNF-beta (human Tumor Necrosis Factor-beta).
Specificity This antibody reacts with Tumor Necrosis Factor beta. Species: Human. Others not tested. Add. Information: Centrifuge vial prior to opening!

Application Details
Alternatives Secondary Antibodies Variants back to top

Application Notes Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity ofhTNF-beta (0.6 ng/ml), a concentration of 0.3 - 0.5 ug/ml of this antibody is required. ELISA: (Indirect): To detect hTNF-β by indirect ELISA (using 100 μl/well antibody solution) aconcentration of 0.5 - 2.0 μg/ml of this antibody is required. This antigen affinity purifiedantibody, in conjunction with compatible secondary reagents, allows the detection of atleast 0.2 - 0.4 ng/well of recombinant hTNF-β. (Sandwich): To detect hTNF-β by sandwich ELISA (using 100 μl/well antibody solution) aconcentration of 0.5 - 2.0 μg/ml of this antibody is required. This antigen affinity purifiedantibody, in conjunction with Biotinylated Anti-Human TNF-β (PP1072B) as a detectionantibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hTNF-β. Western Blot: To detect hTNF-β by Western Blot analysis this antibody can be used at aconcentration of 0.1- 0.2 ug/ml. Used in conjunction with compatible secondary reagentsthe detection limit for recombinant hTNF-β is 1.5-3.0 ng/lane, under either reducingnon-reducing conditions. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Buffer PBS, pH 7.2 without preservatives.Reconstitution: Restore in sterile water to a concentration of 0.1-1.0 mg/ml.
Storage Store the antibody prior to reconstitution at -20°C. Following reconstitution the antibodycan be stored at 2-8°C for one month or at -20°C for longer. Avoid repeated freezing and thawing. Shelf life: One year from despatch.
Research Area Cytokines, Growth Factors, Receptors, Immunology, Inflammation
Restrictions For Research Use only

Alternatives
Application Details Secondary Antibodies Variants back to top

Alternatives for antigen "Lymphotoxin alpha (TNF Superfamily, Member 1) (LTA)", type "Antibodies"
Hosts Rabbit (33), Mouse (5)
Reactivities Human (36), Mouse (Murine) (21), Rat (Rattus) (20), Cow (Bovine) (15), Dog (Canine) (15), Horse (Equine) (15), Rabbit (15)
Applications Immunofluorescence (IF) (22), Flow Cytometry (FACS) (20), Western Blotting (WB) (19), Enzyme Immunoassay (EIA) (7), ELISA (6), Blocking Antibody (Inhibition) (3), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (3), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (2), Immunoprecipitation (IP) (2), Functional Studies (Func) (1), Immunoelectron Microscopy (IEM) (1), Immunohistochemistry (IHC) (1), Neutralization (Neut) (1)
Conjugates Biotin (3), FITC (2), Alexa Flour 488 (1), Alexa Fluor 350 (1), Alexa Fluor 488 (1), Alexa Fluor 555 (1), Alexa Fluor 647 (1), Cy3 (1), Cy5 (1), Cy5.5 (1), Cy7 (1), Gold (1), HRP (1), PE (1), PE,Cy3 (1), PE,Cy5 (1), PE,Cy5.5 (1), PE,Cy7 (1), RBITC (1)
Epitopes Center (1)