BAFF antibody (Biotin)

Catalog No. ABIN116399

The Goat Polyclonal anti-BAFF antibody is suitable to detect BAFF in samples from Human. It has been validated for WB and EIA.

Quick Overview for BAFF antibody (Biotin) (ABIN116399)

Target: BAFF (TNFSF13B) (Tumor Necrosis Factor (Ligand) Superfamily, Member 13b (TNFSF13B))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This BAFF antibody is conjugated to Biotin

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Product Details anti-BAFF Antibody (Biotin)

Specificity: This antibody reacts with BAFF.

Purification: Affinity chromatography

Immunogen: Highly pure (>98%) recombinant hBAFF.

Application Details

Application Notes: ELISA(Direct): To detect hBAFF by direct ELISA (using 100 l/well antibody solution) this antibodycan be used at a concentration of 0.25-1.0 μg/mL. Used in conjunction with compatiblesecondary reagents, allows the detection of at least 0.2-0.4 ng/well of recombinant hBAFF. (Sandwich): To detect hBAFF by sandwich ELISA (using 100 l/well antibody solution) thisantibody can be used at a concentration of 0.25-1.0 μg/mL. Used in conjunction withcompatible secondary reagents, allows the detection of at least 0.2-0.4 ng/well ofrecombinant hBAFF. Western Blot: To detect hBAFF by Western Blot analysis this antibody can be used at a

Restrictions: For Research Use only

Handling

Reconstitution: Centrifuge vial prior to opening. Restore in sterile PBS containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL.

Buffer: PBS, pH 7.2 without preservatives.

Preservative: Without preservative

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody prior to reconstitution at -20 °C. Following reconstitution the antibody can be stored at 2-8 °C for one month or at -20 °C for longer.

Target Details for BAFF

Target: BAFF (TNFSF13B) (Tumor Necrosis Factor (Ligand) Superfamily, Member 13b (TNFSF13B))

Alternative Name: CD257 / BAFF

Background: Members of the TNF superfamily regulate immune responses and induce apoptosis. A novel member in the TNF family was recently identified by several groups and designated BAFF (for B cell Activating Factor belonging to the TNF Family), BLyS (for B Lymphocyte Stimulator), TALL1 (for TNF- and ApoL- related Leukocyte-expressed Ligand), and THANK (for TNF Homologue that Activate Apoptosis, NFkB and c-jun N-terminal Kinase). BAFF/BLyS was characterized as a B cell stimulator since it induced B cell proliferation and immunoglobulin secretion. Two receptors for BAFF were recently identified and designated TACI and BCMA. BAFF also signals through a third TNF receptor BAFFR/BR3. BAFF and its receptors are involved in the development of systemic lupus erythaematosus and other B cell associated autoimmune diseases. Like TNFa and TRAIL, THANK was shown to activate NF-kB and c-jun N terminal kinase (JNK) and to induce apoptosis. The human BAFF gene codes for a 285 amino acid type II transmembrane protein containing a 46 amino acid cytoplasmic domain, a 21 amino acid transmembrane domain, and a 218 amino acid extracellular domain.Synonyms: B cell-activating factor, B lymphocyte stimulator, BAFF, BLYS, Dendritic cell-derived TNF-like molecule, TALL1, TNF- and APOL-related leukocyte expressed ligand 1, TNFSF13B, TNFSF20, Tumor necrosis factor ligand superfamily member 13B, ZTNF4

Gene ID: 10673

NCBI Accession: NP_001139117

UniProt: Q9Y275

Pathways: NF-kappaB Signaling, Production of Molecular Mediator of Immune Response