ALDOA antibody

Catalog No. ABIN116608

This Goat Polyclonal antibody specifically detects ALDOA in WB and EIA. It exhibits reactivity toward Human and Rabbit.

Quick Overview for ALDOA antibody (ABIN116608)

Target: ALDOA (Aldolase A, Fructose-Bisphosphate (ALDOA))

Reactivity: Human, Rabbit

Host: Goat

Clonality: Polyclonal

Conjugate: This ALDOA antibody is un-conjugated

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Product Details anti-ALDOA Antibody

Specificity: Assay by immunoelectrophoresis resulted in a single precipitin arc against purified and partially purified Aldolase [Rabbit Muscle]. This antibody will detect human Aldolase. Cross reactivity against Aldolase from other tissues and species may also occur. It has been reported that this antibody can detect human Aldolase on immunoblot showing a 41 kDa band in lysates from MCF7, NMB231 and HBL100 cell lines.

Purification: Prepared from monospecific antiserum by a delipidation and defibrination.

Immunogen: Aldolase [Rabbit Muscle].

Application Details

Application Notes: Suitable for Immunoblotting (Western or Dot blot), ELISA, Immunoprecipitation and mostImmunological methods requiring high titer and specificity. Recommended Dilutions: This product has been assayed against 1.0 m g of Aldolase[Rabbit Muscle] in a standard sandwich ELISA using Peroxidase conjugated Affinity Purifiedanti-Goat IgG [H&L] (Rabbit) and ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonicacid]) as a substrate for 30 minutes at room temperature. A working dilution of 1: 3,000 to1: 12,000 of the reconstitution concentration is suggested for this product. Useapproximately 5 μL of antibody to immunoprecipitate 50 μL of protein lysate.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Format: Liquid

Reconstitution: Restore with 2.0 mL of deionized water (or equivalent).

Concentration: 90.0 mg/mL (by Refractometry)

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 with 0.01 % sodium azide as preservative.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Dilute only prior to immediate use. Avoid cycles of freezing and thawing.

Storage: -20 °C

Storage Comment: Store vial at -20 °C prior to opening. Centrifuge product if not completely clear after standing at room temperature. For extended storage aliquot contents and freeze at -20 °C or below.

Target Details for ALDOA

Target: ALDOA (Aldolase A, Fructose-Bisphosphate (ALDOA))

Background: Synonyms: ALDA, Fructose-bisphosphate aldolase A, Lung cancer antigen NY-LU-1, Muscle-type aldolase, NY-LU-1

Gene ID: 100009055

NCBI Accession: NP_001075707

UniProt: P00883

Pathways: Ribonucleoside Biosynthetic Process