Bilirubin Oxidase antibody

Catalog No. ABIN116649

The Goat Polyclonal anti-Bilirubin Oxidase antibody (ABIN116649) specifically detects Bilirubin Oxidase in WB and EIA. The antibody is reactive with Myrothecium samples.

Quick Overview for Bilirubin Oxidase antibody (ABIN116649)

Target: Bilirubin Oxidase

Reactivity: Myrothecium

Host: Goat

Clonality: Polyclonal

Conjugate: This Bilirubin Oxidase antibody is un-conjugated

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Product Details anti-Bilirubin Oxidase Antibody

Purification: Prepared from monospecific antiserum by a delipidation and defibrination.

Immunogen: Bilirubin Oxidase [Myrothecium verrucaria].

Application Details

Application Notes: Suitable for Immunoblotting (Western or Dot blot), ELISA, Immunoprecipitation and mostimmunological methods requiring high titer and specificity. Recommended Dilutions: This product has been assayed against 1.0 μg of BilirubinOxidase [Myrothecium verrucaria] in a standard sandwich ELISA using Peroxidaseconjugated Affinity Purified anti-Goat IgG [H&L] (Goat) and ABTS(2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes atroom temperature. A working dilution of 1: 7,500 to 1: 35,000 of the reconstitutionconcentration is suggested for this product.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Format: Liquid

Reconstitution: Restore with 2.0 mL of deionized water (or equivalent).

Concentration: 90.0 mg/mL (by Refractometry)

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 with 0.01 % sodium azide as preservative.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Dilute only prior to immediate use. Avoid cycles of freezing and thawing.

Storage: -20 °C

Storage Comment: Store vial at -20 °C prior to opening. Centrifuge product if not completely clear after standing at room temperature. For extended storage aliquot contents and freeze at -20 °C or below.

Target Details for Bilirubin Oxidase

Target: Bilirubin Oxidase

Background: Bilirubin Oxidase is an enzyme that catalyses the oxidation of bilirubin to biliverdin and other substrates. This enzyme has been expected to be used in the field of clinical pathology for the enzymatic determination of total bilirubin in serum and the treatment of jaundice and hyperbilirubinemia.

Gene ID: 5532

UniProt: Q12737